Subunit respiratory syncytial virus vaccine preparation
AssigneeAventis Pasteur Limited
InventorsGeorge A. Cates, Sonia E. Sanhueza, Raymond P. Oomen, Michel H. Klein
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Abstract
The fusion (F) protein, attachment (G) protein and matrix (M) protein of respiratory syncytial virus (RSV) are isolated and purified from respiratory syncytial virus by mild detergent extraction of the proteins from concentrated virus, loading the protein onto a hydroxyapatite or other ion-exchange matrix column and eluting the protein using mild salt treatment. The F, G and M proteins, formulated as immunogenic compositions, are safe and highly immunogenic and protect relevant animal models against decreased caused by respiratory syncytial virus infection.
Claims22
A mixture of purified fusion (F) protein, attachment (G) protein and matrix (M) protein of respiratory syncytial virus (RSV) in the relative proportions:
- F from about 35 to about 70 wt. %
- G from about 5 to about 30 wt. %
- M from about 10 to about 40 wt. %.
A coisolated and copurified mixture of non-denatured proteins of respiratory syncytial virus (RSV), consisting essentially of the fusion (F) protein, attachment (G) protein and matrix (M) protein of RSV, wherein the mixture is free from lectins and is free from monoclonal antibodies, and wherein said F, G and M proteins are present in the relative proportions:
- F from about 35 to about 70 wt. %
- G from about 5 to about 30 wt. %
- M from about 10 to about 40 wt. %.
A method of producing a coisolated and copurified mixture of proteins of respiratory syncytial virus (RSV), which comprises:
- growing RSV on cells in a culture medium;
- separating the grown virus from the culture medium;
- solubilizing at least the fusion (F) protein, attachment (G) protein and the matrix (M) protein from the separated virus;
- and
- coisolating and copurifying the solubilized RSV proteins to provide a mixture consisting essentially of coisolated and copurified F, G and M proteins.
A method of producing a coisolated and copurified mixture of proteins of respiratory syncytial virus (RSV), which comprises:
- growing RSV on cells in a culture medium;
- separating the grown virus from the cultured medium;
- solubilizing at least the fusion (F) protein, attachment (G) protein and matrix (M) protein from the separated virus;
- and
- coisolating and copurifying the solubilized RSV proteins by:
- loading the solubilized proteins onto an ion-exchange matrix;
- and
- selectively coeluting the F, G and M proteins from the ion exchange matrix.
Dependencies are inferred from the claim text.