Protective epitopes of adenyl cyclase-haemolysin (AC-Hly), their application to the treatment or to the prevention of bordetella infections
AssigneeInstitut Pasteur
InventorsFotini Betsou, Peter Sebo, Nicole Guiso
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Abstract
The subject of the invention is amino acid sequences of the AC-Hly from B. pertussis. B. parapertussis and/or B. bronchiseptica, carrying epitopes capable of inducing a protective immune response against infection by Bordetella. The subject of the invention is antibodies, especially monoclonal antibodies, directed against these epitopes.
Claims34
A purified immunogenic polypeptide of adenyl cyclase-haemolysin (AC-Hly), which induces formation of protective antibodies against an infection by a bacteria selected from the group consisting of B. pertussis, B. parapertussis, and B. bronchiseptica when administered to a human or animal host, and which is selected from the group consisting of:
- a) a polypeptide comprising an amino acid sequence from about amino acid 910 or 913 to the last C-terminal amino acid of AC-Hly from B. pertussis (SEQ ID NO:2) or from B. parapertussis or B. bronchiseptica (SEQ ID NO:4), wherein the polypeptide is modified by addition of a fatty acid between amino acids 980 and 985, provided that the polypeptide does not include the amino acid residues 1-384 of AC-Hly;
- b) a polypeptide consisting of an amino acid sequence from amino acid 385 to amino acid 400 of AC-Hly from B. pertussis (SEQ ID NO:2) or from B. parapertussis or B. bronchiseptica (SEQ ID NO:4);
- c) a polypeptide consisting of an amino acid sequence from amino acid 385 to amino acid 500 of AC-Hly from B. pertussis (SEQ ID NO:2) or from B. parapertussis or B. bronchiseptica (SEQ ID NO:4);
- and
- d) a polypeptide comprising the polypeptides defined above in a) and b), the polypeptides being combined via the chain of amino acids naturally present between the polypeptides defined above in a) and b), within AC-Hly from B. pertussis, B. parapertussis, or B. bronchiseptica, or combined via an antigenic sequence from a protein different from AC-Hly, provided that the polypeptide does not include the amino acid residues 1-384 of AC-Hly.
A purified polypeptide of adenyl cyclase-haemolysin (AC-Hly), which induces formation of protective antibodies against an infection by a bacteria selected from the group consisting of B. pertussis, B. parapertussis, and B. bronchiseptica when administered to a human or animal host, wherein the polypeptide comprises an amino acid sequence from about amino acid 900 or 910 to the last C-terminal amino acid of AC-Hly from B. pertussis (SEQ ID NO:2) or from B. parapertussis or B. bronchiseptica (SEQ ID NO:4), and wherein the polypeptide is modified by addition of a fatty acid between amino acids 980 and 985;
- provided that the polypeptide does not include the amino acid residues 1-384 of AC-Hly.
Dependencies are inferred from the claim text.