Polynucleotide separations on polymeric separation
AssigneeTransgenomic, Inc.
InventorsDouglas T. Gjerde, Paul D. Taylor, Robert M. Haefele
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Abstract
Non-polar polymeric separation media, such as beads or monoliths, are suitable for chromatographic separation of mixtures of polynucleotides when the surfaces of the media are unsubstituted or substituted with a hydrocarbon group having from one to 1,000,000 carbons and when the surfaces are substantially free from mutivalent cation contamination. The polymeric media provide efficient separation of polynucleotides using Matched Ion Polynucleotide Chromatography. Methods for maintaining and storing the polymeric media include treatment with multivalent cation binding agents.
Claims10
A method for separating a mixture of double stranded polynucleotide fragments having up to 1500 base pairs, the method comprising:
- flowing the mixture through a separation column containing polymer beads having an average diameter of 0.5 to 100 microns, said beads being unsubstituted polymer beads or polymer beads substituted with a moiety selected from the group consisting of methyl, ethyl, or hydrocarbon having from 23 to 1,000,000 carbons, wherein said beads are characterized by being substantially free from multivalent cations which are free to bind with DNA, and
- b) separating said mixture of polynucleotides.
Dependencies are inferred from the claim text.