Fatty acid elongases
AssigneesMiami University, The Samuel Roberts Noble Foundation
InventorsJan G. Jaworski, Martha Ann Post-Beittenmiller, James Todd
Life of this patent
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Abstract
Nucleic acids are disclosed that encode fatty acid β-keto acyl synthases from plants. Such synthases are effective for producing very long chain fatty acids (VLCFA), e.g., C22 to C26, preferentially saturated but also monounsaturated. Also disclosed are polypeptides encoded by such nucleic acids. Transgenic plants expressing these polypeptides exhibit altered levels of VLCFA in one or more tissues, such as seeds or leaves.
Claims64
An isolated polynucleotide encoding a polypeptide having an amino acid sequence selected from the group consisting of:
- SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:12, and SEQ ID NO:14.
An isolated polynucleotide, wherein said polynucleotide is selected from the group consisting of:
- a) SEQ ID NO:1;
- b) SEQ ID NO:3;
- c) SEQ ID NO:5;
- d) SEQ ID NO:7;
- e) SEQ ID NO:9;
- f) SEQ ID NO:11;
- g) SEQ ID NO:13;
- h) an RNA analog of SEQ ID NO:1;
- i) an RNA analog of SEQ ID NO:3;
- j) an RNA analog of SEQ ID NO:5;
- k) an RNA analog of SEQ ID NO:7;
- l) an RNA analog of SEQ ID NO:9;
- m) an RNA analog of SEQ ID NO:11;
- n) an RNA analog of SEQ ID NO:13;
- o) a polynucleotide having a nucleic acid sequence complementary to a), b), c), d), e), f), g), h), i), j), k), l), m), or n);
- and
- p) a nucleic acid fragment of a), b), c), f), g), h), i), j), m), n), or o) that is at least 100 nucleotides in length.
A transgenic plant containing a nucleic acid construct comprising a polynucleotide selected from the group consisting of:
- a) SEQ ID NO:1;
- b) SEQ ID NO:3;
- c) SEQ ID NO:5;
- d) SEQ ID NO:7;
- e) SEQ ID NO:9;
- f) SEQ ID NO:11;
- g) SEQ ID NO:13;
- h) an RNA analog of SEQ ID NO:1;
- i) an RNA analog of SEQ ID NO:3;
- j) an RNA analog of SEQ ID NO:5;
- k) an RNA analog of SEQ ID NO:7;
- l) an RNA analog of SEQ ID NO:9;
- m) an RNA analog of SEQ ID NO:11;
- n) an RNA analog of SEQ ID NO:13;
- o) a polynucleotide having a nucleic acid sequence complementary to a), b), c), d), e), f), g), h), i), j), k), l), m), or n);
- and
- p) a nucleic acid fragment of a), b), c), f), g), h), i), j), m), n), or o) that is at least 100 nucleotides in length.
A transgenic plant containing a nucleic acid construct comprising a polynucleotide encoding a polypeptide having an amino acid sequence selected from the group consisting of:
- SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, and SEQ ID NO:14.
A method of altering the levels of very long chain fatty acids in a plant, comprising the steps of:
- a) creating a nucleic acid construct, said construct comprising a polynucleotide selected from the group consisting of:
- a) SEQ ID NO:1;
- b) SEQ ID NO:3;
- c) SEQ ID NO:5;
- d) SEQ ID NO:7;
- e) SEQ ID NO:9;
- f) SEQ ID NO:11;
- g) SEQ ID NO:13;
- h) an RNA analog of SEQ ID NO:1;
- i) an RNA analog of SEQ ID NO:3;
- j) an RNA analog of SEQ ID NO:5;
- k) an RNA analog of SEQ ID NO:7;
- l) an RNA analog of SEQ ID NO:9;
- m) an RNA analog of SEQ ID NO:11;
- n) an RNA analog of SEQ ID NO:13;
- o) a polynucleotide having a nucleic acid sequence complementary to a), b), c), d), e), f), g), h), i), j), k), l), m), or n);
- and
- p) a nucleic acid fragment of a), b), c), f), g), h), i), j), m), n), or o) that is at least 100 nucleotides in length;
- and
- b) introducing said construct into said plant, wherein said polynucleotide is effective for altering the levels of very long chain fatty acids in said plant.
An isolated polynucleotide encoding a polypeptide having the amino acid sequence of SEQ ID NO:8.
An isolated polynucleotide encoding a polypeptide having the amino acid sequence of SEQ ID NO:10.
Dependencies are inferred from the claim text.