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US 6,307,127 B1Granted
Transformation of guar
AssigneeDanisco A/S
InventorsMorten Jørsboe, Finn T. Okkels
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AssigneeDanisco A/S
InventorsMorten Jørsboe, Finn T. Okkels
Estimating the term…
The invention relates to a method for the transformation of legumes of the genus Cyamopsis, in particular Agrobacterium-mediated transformation of guar (Cyamopsis tetragonoloba), by introducing a recombinant DNA sequence into at least one cell or protoplast and generating genetically modified explants using at least one selection or shoot growth medium comprising at least one compound selected from an auxin inhibitor, e.g. 2-(p-chlorophenoxy)-2-methylpropionic acid (PCIB), a β-lactamase inhibitor, e.g. sulbactam, and an ethylene inhibitor, e.g. silver thiosulfate, so as to obtain genetically modified plant or part thereof containing in its genome at least one recombinant DNA sequence; to genetically modified plants produced by the method; and to the use of substances such as the β-lactamase inhibitor sulbactam to facilitate transformation of guar and other plants.
A method for producing a genetically modified plant or part thereof of the genus Cyamopsis, comprising the steps of introducing a recombinant DNA sequence into at least one cell or protoplast by means of a β-lactamase producing agrobacterium and generating genetically modified explants using at least one selection or shoot growth medium comprising (a) at least one β-lactamase inhibitor and (b) at least one auxin inhibitor or ethylene inhibitor, so as to obtain a genetically modified plant or part thereof containing in its genome at least one recombinant DNA sequence.
A method for producing a genetically modified plant or part thereof of the genus Cyamopsis, comprising the steps of introducing a recombinant DNA sequence into at least one cell or protoplast by means of a β-lactamase producing agrobacterium and generating genetically modified explants using at least one selection or shoot growth medium comprising a nickel salt, so as to obtain a genetically modified plant or part thereof containing in its genome at least one recombinant DNA sequence.
A method for producing a genetically modified plant or part thereof of the genus Cyamopsis, comprising the steps of introducing a recombinant DNA sequence into at least one cell or protoplast by means of a β-lactamase producing Agrobacterium and generating genetically modified explants using at least one selection or shoot growth medium comprising at least one β-lactamase inhibitor.
Dependencies are inferred from the claim text.