Nucleic acid molecules encoding enzymes from wheat which are involved in starch synthesis
AssigneeHoechst Schering AgrEvo GmbH
InventorsMartina Block, Horst Lörz, Stephanie Lütticke, Lennart Walter, Claus Frohberg, Jens Kossmann
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Abstract
The present invention relates to nucleic acid molecules encoding enzymes which are involved in the starch synthesis in plants. These enzymes are starch synthases from wheat. The invention further relates to vectors and host cells containing said nucleic acid molecules, in particular transformed plant cells and plants regenerated from these cells, which exhibit an increased or a reduced activity of the described starch synthases.
Claims29
An isolated nucleic acid molecule comprising a nucleic acid sequence encoding a protein with a biological activity of a starch synthase, wherein the nucleic acid sequence is selected from the group consisting of:
- (a) a nucleic acid sequence encoding a protein comprising the amino acid sequence of SEQ ID NO:2;
- (b) a nucleic acid sequence comprising the coding region of SEQ ID NO:
- 1;
- (c) a nucleic acid sequence that has at least 60% sequence identity to the entire coding region of the nucleic acid sequence of (a) or (b) and that encodes a protein with a biological activity of a soluble starch synthase;
- (d) a nucleic acid sequence that deviates from the sequence of any one of the nucleic acid sequences of (a), (b) or (c) due to the degeneracy of the genetic code;
- (e) a nucleic acid sequence encoding a protein which comprises the amino acid sequence of SEQ ID NO:6;
- (f) a nucleic acid sequence comprising the coding region of SEQ ID NO:5;
- (g) a nucleic acid sequence that has at least 60% sequence identity to the entire coding region of the nucleic acid sequence of (e) or (f) and that encodes a protein with a biological activity of a granule bound starch synthase;
- and
- (h) a nucleic acid sequence that deviates from the sequence of any one of the sequences of (e), (f), or (g) due to the degeneracy of the genetic code.
An isolated nucleic acid molecule comprising a nucleotide sequence encoding a protein with a biological activity of a starch synthase, wherein the nucleic acid sequence is more than 90% homologous to:
- (a) the coding region of SEQ ID NO:
- 1;
- or
- (b) the coding region of SEQ ID NO:5.
An isolated nucleic acid molecule comprising a nucleic acid sequence encoding a protein with a biological activity of a starch synthase, wherein the nucleic acid sequence has at least 60% sequence identity to:
- (a) the entire coding region of SEQ ID NO:
- 1;
- or
- (b) the entire coding region of SEQ ID NO:
- 5.
Dependencies are inferred from the claim text.