Methods and compositions for transgene identification

A method of identifying transgenic seeds comprising the steps of:

• (a) obtaining a transgenic plant, the cells of which comprise a gene fusion between a selectable marker gene and a screenable marker gene, wherein said gene fusion is operably linked to an aleurone-specific promoter;
• (b) cultivating said transgenic plant until seed set;
• (c) collecting seeds produced on said transgenic plant;
• and
• (d) screening said seeds based on a phenotype conferred upon the seeds by said screenable marker gene.

A method of plant breeding comprising the steps of:

• (a) obtaining first and second plants, wherein cells of one or both of said first or second plants comprise a gene encoding a screenable marker operably linked to an aleurone-specific promoter, wherein said screenable marker gene is fused to a selectable marker gene and wherein said screenable marker is further defined as capable of detection by non-destructive assay;
• (b) crossing said first and second plants;
• and
• (c) collecting the seeds resulting from said crossing.

A method of plant breeding comprising the steps of:

• (a) obtaining a transgenic plant, the cells of which comprise a gene encoding a screenable marker operably linked to an aleurone-specific promoter, wherein said screenable marker gene is fused to a selectable marker gene and wherein said screenable marker is further defined as capable of detection by non-destructive assay;
• (b) allowing said plant to self fertilize;
• and
• (c) collecting the seeds produced on said plant.