Circular, hairpin, circular/hairpin, lariat, and hairpin-lariat hammerhead ribozymes
AssigneeUniversity of Utah Research Foundation
InventorsDuane E. Ruffner, Laixin Wang
Life of this patent
Estimating the term…
Abstract
Hammerhead ribozyme derivatives termed circular, hairpin, circular/hairpin, lariat, and hairpin-lariat ribozymes are disclosed. These hammerhead ribozyme derivatives are catalytically active and have increased specific activity and reduced requirement for divalent metal ion co-factor as compared to wild type hammerhead ribozyme.
Claims19
A hammerhead ribozyme derivative having a structure represented by:
- (L)b—R1-(cugauga)-R2
- wherein R1 and R2 are oligoribonucleotides of at least 2 bases configured for base pairing with a substrate;
- L is a spacer, and b is 0 or 1, with the proviso that if b is 0, then R1 and R2 are bonded together with a phosphodiester bond, and if b is 1, then L is bonded to R2.
A hammerhead ribozyme derivative having a structure represented by:
- R3—(L1)b—R1-(cugauga)-R2—(L2)d—R4
- wherein R1 and R2 are oligoribonucleotides of at least 2 bases configured for base pairing with a substrate;
- R3 and R4 are oligonucleotides of at least 2 bases configured for base pairing with each other, L1 and L2 are spacers;
- and b and d are 0 or 1.
A hammerhead ribozyme derivative having a structure represented by:
- R3—(L1)b—R1-(cugauga)-R2—(L2)d—R4—(L3)f
- wherein R1 and R2 are oligoribonucleotides of at least 2 bases configured for base pairing with a substrate;
- R3 and R4 are oligonucleotides of at least 2 bases configured for base pairing with each other;
- L1, L2, and L3 are spacers;
- and b, d, and f are 0 or 1, with the proviso that if f is 0, then R3 and R4 are bonded together by a phosphodiester bond, and if f is 1, then L3 is bonded to both R3 and R4.
A hammerhead ribozyme derivative having a structure represented by:
- (L1)b—R1-(cugauga)-R2—Q—(L2)d—R4
- wherein R1 is an oligoribonucleotide of at least 2 bases configured for base pairing with a substrate;
- R2 is an oligoribonucleotide of at least 2 bases configured for base pairing with R4;
- R4 is an oligonucleotide of at least 2 bases configured for base pairing with R2;
- L1 and L2 are spacers;
- Q is a branching moiety;
- and b and d are 0 or 1, with the proviso that is b is 0, then R1 is covalently bonded to Q, and if b is 1, then L1 is covalently bonded to Q.
A hammerhead ribozyme derivative having a structure represented by:
- R3—(L1)b—R1-(cugauga)-R2—(L2)d—R4
- wherein R1 is an oligoribonucleotide of at least 2 bases configured for base pairing with a substrate;
- R2 is an oligoribonucleotide of at least 2 bases configured for base pairing with both R3 and R4;
- R3 is an oligonucleotide of at least 2 bases configured for base pairing with R2;
- L1 and L2 are spacers;
- and b and d are 0 or 1.
Dependencies are inferred from the claim text.