BRCA1 associated polynucleotide (BAP-1) and uses therefor
AssigneeThe Wistar Institute of Anatomy and Biology
InventorsFrank J. Rauscher, III, David E. Jensen
Life of this patent
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Abstract
Nucleic acid and amino acid sequences of a BRCA1 associated protein, BAP-1, are provided. These sequences, the protein, and anti-BAP-1 antibodies are useful in therapeutics and diagnostics for cancers associated with loss of the 3p21 chromosomal region and/or inappropriate BAP-1 levels.
Claims11
A nucleic acid sequence encoding mammalian BRCA1 Associated Protein (BAP-1) comprising nucleotides 40-2226 of SEQ ID NO:
- 1, or a sequence completely complementary thereto, isolated from cellular materials with which it is naturally associated.
An isolated fragment of a nucleic acid sequence encoding mammalian BRCA1 Associated Protein (BAP-1), wherein said fragment is selected from the group consisting of:
- (a) an interactive domain, nucleotides 1831 to 2226 of SEQ ID NO:
- 1;
- and
- (b) a sequence completely complementary to the sequence of (a).
A recombinant molecule including a mammalian nucleic acid sequence encoding a BRCA1 associated protein (BAP-1) or peptide, said nucleic acid sequence comprising nucleotides 40-2226 of SEQ ID NO:
- 1 under the control of regulatory sequences, which permit expression of the BAP-1 protein or peptide in a host cell.
A method of recombinantly expressing BRCA1 associated protein (BAP-1) comprising culturing a recombinant host cell transformed with a nucleic acid sequence comprising nucleotides 40-2226 of SEQ ID NO:
- 1 under conditions which permit expression of BAP-1.
A reagent including an isolated or recombinant nucleic acid sequence selected from the group consisting of:
- (a) SEQ ID NO:
- 1;
- (b) a nucleotide sequence containing nucleotides 40 to 2226 of SEQ ID NO:
- 1;
- (c) a nucleotide sequence consisting of a nucleotide sequence encoding amino acids 598 to 729 of SEQ ID NO:
- 2;
- (d) a sequence completely complementary to a sequence of (a) through (c);
- and a detectable label which is bound to said sequence.
A composition comprising an isolated or recombinant nucleic acid molecule selected from the group consisting of
- (a) a plasmid comprising a nucleic acid sequence encoding a BAP-1 protein or peptide, said nucleic acid sequence comprising nucleotides 40-2226 of SEQ ID NO:
- 1 under the control of regulatory sequences directing expression of the encoded protein or peptide in a host cell;
- (b) a virus vector comprising a nucleic acid sequence encoding a BAP-1 protein or peptide, said nucleic acid sequence comprising nucleotides 40-2226 of SEQ ID NO:
- 1 under the control of regulatory sequence directing expression of the encoded protein or peptide in a host cell;
- and a pharmaceutically acceptable carrier.
A recombinant molecule including a nucleic acid sequence selected from the group consisting of
- (a) SEQ ID NO:
- 1;
- (b) a nucleotide sequence containing nucleotides 40 to 2226 of SEQ ID NO:
- 1;
- (c) a nucleotide sequence consisting of a nucleotide sequence encoding amino acids 598 to 729 of SEQ ID NO:
- 2;
- and
- (d) a sequence completely complementary to a sequence of (a) through (c);
- and a detectable label.
A plasmid comprising a nucleic acid sequence encoding a BAP-1 protein or peptide, said nucleic acid sequence comprising nucleotides 40-2226 of SEQ ID NO:
- 1 under the control of regulatory sequences directing expression of the encoded protein or peptide in a host cell.
A virus vector comprising a nucleic acid sequence encoding a BAP-1 protein or peptide, said nucleic acid sequence comprising nucleotides 40-2226 of SEQ ID NO:
- 1 under the control of regulatory sequence directing expression of the encoded protein or peptide in a host cell.
Dependencies are inferred from the claim text.