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US 6,307,031 B1Granted
Beta taipoxin as a cell growth factor and method
OwnerBinie V. Lippsinventor
Life of this patent
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Abstract
A peptide containing a sequence for the first fifteen amino acids from the N-terminal of Asn-Leu-Val-Glu-Phe-Gly-Lys-Met-Ile-Glu-Cys-Ala-Ile-Arg-Asn is used in a cell culture medium to promote cell growth in vitro and in the treatment of wounds. A method of preparation of the peptide is also claimed.
Claims4
A method for preparing beta taipoxin from taipan snake venom in a single pass through a high pressure liquid chromatograph, said method comprising:
- diluting the taipan snake venom with phosphate buffer saline,
- separating a supernatant liquid from the diluted venom, and
- fractionating the supernatant liquid on a high pressure liquid chromatograph, using an ion exchange column and a gradient buffer consisting essentially of (2-amino-2-(hydroxymethyl)propane-1,3-diol)-HCl and water and having a pH in the range of 6.0-8.0 to elute a venom fraction consisting essentially of beta taipoxin.
A method for separating beta taipoxin from a snake venom fraction which comprises beta taipoxin together with alpha taipoxin and gamma taipoxin, said method comprising
- fractionating the snake venom fraction on a high pressure liquid chromatograph, using an ion exchange column and a gradient buffer consisting essentially of (2-amino-2-(hydroxymethyl)propane-1,3-diol)-HCl and water and having a pH in the range of 6.0-8.0 to elute the beta taipoxin separately from the alpha taipoxin and the gamma taipoxin.
Dependencies are inferred from the claim text.