Alkaline lipase and detergent composition active at low temperature
AssigneeNovozymes A/S
InventorMasahiro Suzuki
Life of this patent
Estimating the term…
Abstract
Lipases having improved washing performance for use in detergents to improve the removal of fatty soiling, and having activity throughout a temperature range of 1 to 65° C., including an activity at 1° C. which is at least 20% the activity at 30° C., activity over a pH range of 8-12, and activity at high concentration of anionic surfactant and in the presence of bleach.
Claims16
A lipase obtained from P. Stutzeri strain SD711 (FERM BP-5892), SD712 (FERM BP-5893), or SD713 (FERM BP-5894);
- Pseudomonas sp. strain SD714 (FERM BP-5895), SD715 (FERM BP-5896), SD716 (FERM BP-5897), or SD717 (FERM BP-5898), or Acinetobacter, wherein said lipase has the following properties:
- a) an optimum temperature of at least 40° C.;
- and
- b) an activity at 1° C. which is at least 20% of the activity at 30° C.
A lipase obtained from P. Stutzeri strain SD711 (FERM BP-5892), SD712 (FERM BP-5893), or SD713 (FERM BP-5894);
- Pseudomonas sp. strain SD714 (FERM BP-5895), SD715 (FERM BP-5896), SD716 (FERM BP-5897), or SD717 (FERM BP-5898), or Acinetobacter, which has an activity measured in a solution containing 0.02% sodium linear alkylbenzene sulfonate (LAS) using an olive oil emulsion as a substrate which is at least 20% of the activity in the absence of LAS, and which has at least one of the following properties a) to c) below:
- a) a decomposition rate of p-nitrophenyl palmitate in a solution containing 0.046% LAS at 25° C. in the presence of 0.5% hydrogen peroxide of at least 80% of the decomposition rate in the absence of hydrogen peroxide;
- b) a residual activity after incubation in a solution containing 0.05% LAS and 0.55% hydrogen peroxide at 25° C. for 1 hour, which is at least 50% of the activity before the incubation;
- and/or
- c) a decomposition rate of p-nitrophenyl palmitate in a solution at 25° C. containing 0.2% LAS which is at least 50% of the decomposition rate in a solution containing 0.02% LAS.
A lipase obtained from P. Stutzeri strain SD711 (FERM BP-5892), SD712 (FERM BP-5893), or SD713 (FERM BP-5894);
- Pseudomonas sp. strain SD714 (FERM BP-5895), SD715 (FERM BP-5896), SD716 (FERM BP-5897), or SD717 (FERM BP-5898), or Acinetobacter and which has property a) and/or b) below:
- a) a decomposition rate of p-nitrophenyl palmitate in a solution at 25° C. containing 0.046% LAS in the presence of 0.5% hydrogen peroxide which is at least 80% of the decomposition rate in the absence of hydrogen peroxide;
- and
- b) a residual activity after incubation in a solution containing 0.05% LAS and 0.55% hydrogen peroxide at 25° C. for 1 hour, which it at least 50% of the initial activity before the incubation.
A lipase obtained from P. Stutzeri strain SD711 (FERM BP-5892), SD712 (FERM BP-5893), or SD713 (FERM BP-5894);
- Pseudomonas sp. strain SD714 (FERM BP-5895), SD715 (FERM BP-5896), SD716 (FERM BP-5897), or SD717 (FERM BP-5898), or Acinetobacter, which has the following properties:
- a) activity throughout a pH range of 8.0 to 2.0 and an optimum pH within a range of 8.5 to 12;
- b) activity throughout a temperature range of 1 to 65° C. and an optimum temperature within a range of 45 to 65° C.;
- c) a molecular weight of 30,000±3,500 as measured by SDS polyacrylamide gel electrophoresis.
A bacterium selected from the group consisting of P. Stutzeri strain SD711 (FERM BP-5892), SD712 (FERM BP-5893), or SD713 (FERM BP-5894);
- Pseudomonas sp. strain SD714 (FERM BP-5895), SD715 (FERM BP-5896), SD716 (FERM BP-5897), or SD717 (FERM BP-5898).
A lipase which has the following properties:
- a) an optimum temperature of at least 40° C.;
- b) an optimum pH greater than 11;
- and
- c) an activity at 1° C. which is at least 20% of the activity at 30° C.
A lipase which has the following properties
- a) an optimum temperature of less than 55° C. but at least 40° C.;
- and
- b) an activity at 1° C. which is at least 20% of the activity at 30° C.
Dependencies are inferred from the claim text.