Covalent bonding of molecules to an activated solid phase material
AssigneeA-Fem Medical Corporation
InventorsRobert Lee Buck, Huiying Wang, Timothy Patrick Hyatt, Paul Andrew Mueggler
Life of this patent
Estimating the term…
Abstract
Microporous solid phase materials that are suitable for lateral flow and other assays for detecting the presence of analytes in test samples, that are stable under variations in humidity and, even after storage for extended periods of time, can form stable covalent bonds with molecules containing a free primary or secondary amine group or sulfhydryl group are described. The invention further concerns chemically derivatized solid phase materials, and conjugates comprising such materials. Examples of lateral flow devices for the quantitative or semi-quantitative determination of an analyte in a biological sample are described.
Claims16
A conjugate having the formula
- where X is a hydroxyl bearing solid phase material;
- R5 is (CH2)n(NH(CH2)m)p, where n is from 2 to 8, m is from 2 to 8, and p is from 0 to 3;
- R6 is selected from the group consisting of alkyl, cyclic alkyl, aromatic and heterocyclic groups containing 0 to 8 hydroxyl, hydroxycarbonyl, or aminocarbonyl groups;
- and Q is any molecule that contains a free primary or secondary amine group.
Dependencies are inferred from the claim text.