Parallel production of high density arrays
AssigneeUnitec S.P.A.
InventorDon Stimpson
Life of this patent
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Abstract
A method to produce arrays of compounds for concurrent testing is described. Two formats are described using porous rods or porous sheet materials. In one format, the compounds of the array are immobilized onto porous rod elements. In the second format, the compounds are immobilized as lines on a sheet of porous material. In both cases, a bundle is formed by radial compression of the rods or spiral wrapping of the sheet. A sheath is applied to the bundle, and arrays are cut as slabs. Each synthesis or application step to create an array element is used to fabricate multiple arrays. Relatively high-density arrays can be produced with current printing technologies. The method is particularly suited to mass production of arrays.
Claims22
A method for detection of components in a sample mixture or detection of components on an array comprising the steps of:
- a. immobilizing binding compounds specific for detecting the components onto a plurality of carrier arrays;
- b. forming a bundle in a generally elongated form by collecting the plurality of carrier arays about a common axis;
- c. cutting individual arrays from the bundle to generate a spatially uniform arrangement of binding compounds on said arrays;
- d. contacting the components with the binding compounds to form bound components;
- and
- e. detecting a presence of bound components.
A method for detection of compounds on an array by:
- a. immobilizing binding compounds onto porous rod shaped array elements;
- b. forming a bundle of the rod shaped array elements using a guide to create a spatially uniform arrangement of rod shaped array elements and securing with a sheath material;
- c. cutting individual rod shaped array elements from the bundle to generate a multiplicity of binding surfaces and fixing or placing one side of the rod shape element array to an absorbent pad;
- d. applying a sample to a surface of an element and allowing the sample to flow through the porous rod elements into the absorbent pad;
- e. exposing and developing the array elements to detect the compounds;
- and
- f. correlating a binding pattern of the binding surfaces with a composition of array elements.
A method of detecting components in a sample mixture or detection of compounds on an array by:
- a. forming lines of immobilized binding compounds on a sheet of material, wherein lines of binding compounds are separated by identification marks, and the binding material is impregnated into a thickness of the sheet material;
- b. rolling the sheet of material into spiral wound structure about a rod to form a bundle of arrays and securing said bundle with a sheath;
- c. cutting individual arrays from the bundle to expose binding elements formed by a freshly exposed edge of sheet material separated by identification marks and interstitial spaces between adjacent membrane layers, and fixing or placing one side of the array onto an absorbent pad;
- d. contacting the components with the array, and exposing and developing the array to detect the components;
- and
- e. correlating a binding pattern on the array with a composition of the binding compounds.
A method of detecting compounds on an array by:
- a. a synthesizing of binding compounds onto rod shaped array elements using a randomized method;
- b. marking an identification of each rod shaped array element at each synthesis step by spin coating with paint;
- c. forming a bundle of the rod shaped array elements and securing with a sheath material;
- d. cutting individual arrays from the bundle;
- e. exposing the array elements to a sample mixture and developing the array to form binding events;
- and
- f. correlating binding events with the identification marks to detect a sequence of bound compounds.
A method of detecting components in a sample mixture or detection of compounds on an array by:
- a. immobilizing binding compounds onto rod shaped array elements;
- b. forming a bundle of the rod shaped array elements and securing with a sheath material so that rod shaped array elements treated with a given binding agent are grouped to create a graphic symbol surrounded by rod shaped array elements with different or substantially no affinity for components in the sample mixture;
- c. cutting individual array elements from the bundle and fixing or placing one side of the array element to an absorbent pad;
- d. contacting the components with the array element, and exposing and developing the array element;
- e. correlating the graphic symbol with a presence or absence of the components thereby detecting the components.
Dependencies are inferred from the claim text.