Container for explant culture, method of using same, and process, mold and apparatus of making same
AssigneeIncell Co., Ltd.
InventorsAlexander Tanklevsky, Michael Tanklevsky, Alla Tanklevsky
Life of this patent
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Abstract
An improved device, in the form of a container, for propagating explant cultures under sterile conditions while facilitating gas exchange with an outside environment, methods for employing the device, a process of manufacture to produce the device, a mold for use as part of the process of manufacture, and an apparatus for conducting the process of manufacture employing the mold.
Claims3
A device for propagating explant cultures, the device comprising:
- (a) at least one growth chamber for containing and retaining therein a culture medium;
- and
- (b) a gas permeable, liquid impermeable membrane sealing said at least one growth chamber;
- wherein said at least one growth chamber includes a plurality of growth chambers, whereas said plurality of growth chambers are in fluid communication with one another through medium non-containing portions thereof.
An assembly comprising a plurality of units each of said plurality of units including a device for propagating explant cultures, the device comprising:
- (a) at least one growth chamber for containing and retaining therein a culture medium;
- and
- (b) a gas permeable, liquid impermeable membrane sealing said at least one growth chamber;
- wherein said at least one growth chamber retains a plant support matrix selected from the group consisting of agar, agarose, a polyurethane foam, a non-woven polypropylene fiber, glass beads, a microporous polypropylene membrane, a polypropylene netting, polyacrylamide beads, super absorbent gel, vermiculite and soil,
- wherein at least some of said plurality of units are separated therebetween via perforations.
A device for propagating explant cultures, the device comprising:
- (a) at least one growth chamber for containing and retaining therein a culture medium;
- and
- (b) a gas permeable, liquid impermeable membrane sealing said at least one growth chamber, constructed such that each of said at least one growth chamber is adapted for holding a liquid therein when vertically positioned and when said gas permeable, liquid impermeable membrane, is yet unsealed thereto.
Dependencies are inferred from the claim text.