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US 6,306,642 B1Granted
Enzyme substrate delivery and product registration in one step enzyme immunoassays
AssigneeQuidel Corporation
InventorsAlan M. Nelson, Jan W. Pawlak, Allan D. Pronovost
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Abstract
One-step enzyme immunoassays in which enzyme-antibody conjugate or label and enzyme substrate are separated until separation of bound and free enzyme conjugate or label is complete. This separation is accomplished by using variable flow paths, immobilization of substrate at the test line, placement of substrate in a sac or association with a particle label, enzyme product chemical capture, delay zone dissolution and protected enzyme substrates.
Claims4
An enzyme immunoassay device, comprising:
- a sample pad comprising a first lane containing a first barrier zone and a second lane containing a second barrier zone, wherein said first lane contains an enzyme-antibody conjugate having affinity for an analyte and said second lane contains an enzyme substrate, wherein said first barrier zone dissolves before said second barrier zone;
- a capture zone in fluid communication with said sample pad, said capture zone containing a capture antibody incorporated therein having affinity for said analyte;
- and
- an absorbent zone in fluid communication with said capture zone.
1 more element
Dependencies are inferred from the claim text.