Genetically modified cyanobacteria for the production of ethanol, the constructs and method thereof
AssigneeEnol Energy Inc.
InventorsRobert Paul Woods, John Robert Coleman, Ming De Deng
Life of this patent
Estimating the term…
Abstract
The invention relates to the genetic modification of Cyanobacteria for the production of ethanol, and more particularly, to the genetic modification of Cyanobacteria by incorporating the genetic information encoding for pyruvate decarboxylase (pdc) and alcohol dehydrogenase (adh).
Claims11
A construct comprising an rbcLS promoter of Synechococcus and a lacZ promoter of Escherichia coli operatively linked to DNA fragments encoding pdc and adh enzymes obtained from Zymomonas mobilis.
A construct comprising an rbcLS promoter of Synechococcus and a lacZ promoter of Escherichia coli operatively linked to DNA encoding pdc and adh enzymes obtained from Zymomonas mobilis.
A construct selected from the group consisting of pCB4-Rpa, pCB4-LRpa, pCB4-LR(TF)pa and pCB4-CPpa.
Genetically modified Cyanobacteria containing a temperature inducible construct comprising DNA fragments encoding pyruvate decarboxylase (pdc) and alcohol dehydrogenase (adh) enzymes obtained from the Zymomonas mobilis plasmid pLOI295 wherein said construct is pCB4-CPpa and said Cyanobacteria are capable of producing ethanol photosynthetically via a carbohydrate reserve.
Genetically modified Cyanobacteria containing a construct comprising DNA fragments encoding pyruvate decarboxylase (pdc) and alcohol dehydrogenase (adh) enzymes obtained from the Zymomonas mobilis plasmid pLOI295, said Cyanobacteria being capable of producing ethanol photosynthetically via a carbohydrate reserve and are produced according to the following steps:
- a. selecting an appropriate promoter;
- b. ligating said promoter to pdc and adh encoding DNA sequences;
- c. cloning said ligated sequences comprising the promoter, pdc and adh sequences into an appropriate construct, said construct selected from the group consisting of pCB4-Rpa, pCB4-LRpa and pCB4-LR(TF)pa;
- d. transforming said construct into said Cyanobacteria,
- wherein said Cyanobacteria is Synechococcus PCC 7942 or other transformable strains of Synechococcus.
Dependencies are inferred from the claim text.