Nucleic acid segments encoding wheat acetyl-CoA carboxylase
AssigneeArch Development Corporation
InventorsRobert H. Haselkorn, Piotr Gornicki
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Abstract
The present invention provides isolated and purified polynucleotides that encode plant polypeptides that participate in the carboxylation of acetyl-CoA. Also provided are methods for identifying such nucleic acid segments and polypeptides. Processes for altering acetyl-CoA carboxylation, increasing herbicide resistance of plants and identifying herbicide resistant variants of acetyl-CoA carboxylase are also provided.
Claims28
A purified nucleic acid segment having the nucleic acid sequence of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7 or SEQ ID NO:8;
- or a nucleic acid segment encoding a wheat acetyl-CoA carboxylase comprising the amino acid sequence of SEQ ID NO:9.
A purified nucleic acid segment having the nucleic acid sequence of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7 or SEQ ID NO:8;
- or a nucleic acid segment encoding a wheat acetyl-CoA carboxylase comprising the amino acid sequence of SEQ ID NO:9, wherein said segment comprises a null mutation.
A method of producing an acetyl CoA-carboxy polypeptide comprising:
- (a) introducing into a host cell a nucleic acid segment having a nucleic acid sequence of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7 or SEQ ID NO:8;
- and
- (b) culturing said host cell under conditions permitting transcription and translation of said acetyl CoA-carboxylase polypeptide from said nucleic acid sequence.
Dependencies are inferred from the claim text.