Fungal cellulases
AssigneeGist-Brocades N.V.
InventorsHenriëtte C. Van Den Broeck, Leendert H. De Graaff, Jacob Visser, Albert J. J. Van Ooijen
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Abstract
The present invention provides polypeptides with cellulase activity, corresponding DNA sequences, vectors, transformed hosts and a method for the production of these polypeptides, which are obtainable from Aspergillus niger var. niger or Aspergillus niger var. tubigensis.
Claims16
An isolated nucleic acid fragment comprising
- (a) a sequence encoding a polypeptide having carboxymethyl cellulase (CMCase), endoglucanase, and β-glucanase activity and being indigenous to Aspergillus niger var. niger or Aspergillus niger var. tubigensis;
- (b) a sequence encoding a polypeptide having the amino acid sequence SEQ ID NO:2 or SEQ ID NO:4 or a variant thereof that differs by an addition, insertion or deletion of one or more amino acids, and which still has CMCase, endoglucanase and β-glucanase activity;
- wherein said variant has an amino acid sequence that has at least 70% sequence identity to the polypeptides of SEQ. ID. NO:2 or SEQ. ID. NO:4;
- or
- (c) a sequence shown in SEQ ID NO:1 or 3, or sequence having at least 90% sequence identity to the sequence shown in SEQ ID NO:1 or 3 and encoding a peptide having CMCase, endoglucanase, and β-glucanase activity.
An isolated nucleic acid fragment encoding a polypeptide having carboxymethyl cellulase (CMCase), endoglucanase and β-glucanase activity and comprising the nucleic acid sequence shown in SEQ ID NO:1 or 3, or a nucleic acid sequence having at least 90% sequence identity to the sequence shown in SEQ ID NO:1 or 3.
Dependencies are inferred from the claim text.