Esterase gene and its use
AssigneeSumitomo Chemical Company, Limited
InventorsTakeshi Ishii, Satoshi Mitsuda
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Abstract
An isolated esterase gene coding for an esterase capable of causing asymmetric hydrolysis of an organic carboxylic acid ester of a cyclopentenolone of formula I; wherein R1 is C1-C10 alkyl, C2-C10 alkenyl, C2-C10 alkynyl or C1-C4 haloalkyl, to produce the cyclopentenolone of formula I in (S)-form, and hybridizing to the base sequence of SEQ ID NO:1, is useful for the industrially favorable production of optically active cyclopentenolones of formula I which serve as the intermediates of drugs, agricultural chemicals or other active products.
Claims7
An isolated polynucleotide coding for an esterase capable of causing asymmetric hydrolysis of an organic carboxylic acid ester of a cyclopentenolone of formula I:
- wherein R1 is C1-C10 alkyl, C2-C10 alkenyl, C2-C10 alkynyl or C1-C4 haloalkyl, to produce the cyclopentenolone of formula I in (S)-form, and having:
- (a) a base sequence of SEQ ID NO:1,
- (b) a base sequence coding for the amino acid sequence of SEQ ID NO:2, or
- (c) a base sequence of SEQ ID NO:1 with the addition, deletion or substitution of one or more bases, wherein when a double-stranded DNA of said base sequence is dissociated into complementary single-stranded DNAs by heat treatment at 95° C. for 1 minute or alkali treatment with 0.5 M NaOH, 1.5 M NaCl, said single-stranded DNAs hybridize with the base sequence of SEQ ID NO:1 when cooled ice for 1 minute or subjected to neutralization with 0.5 M Tris-HCl, pH 7.0, 3.0 M NaCl, and wherein the homology as a whole, of the polynucleotide is 90% to SEQ ID NO:
- 1 or higher.
An isolated polynucleotide coding for an esterase capable of causing asymmetric hydrolysis of an organic carboxylic acid ester of a cyclopentenolone of formula I:
- wherein R1 is C1-C10 alkyl, C2-C10 alkenyl, C2-C10 alkynyl or C1-C4 haloalkyl, to produce the cyclopentenolone of formula I in (S)-form, and having a base sequence coding for the amino acid sequence of SEQ ID NO:2.
An isolated polynucleotide coding for an esterase capable of causing asymmetric hydrolysis of an organic carboxylic acid ester of a cyclopentenolone of formula I:
- wherein R1 is C1-C10 alkyl, C2-C10 alkenyl, C2-C10 alkynyl or C1-C4 haloalkyl, to produce the cyclopentenolone of formula I in (S)-form, and having the base sequence of SEQ ID NO:1.
Dependencies are inferred from the claim text.