Method for obtaining expression of mixed polypeptide particles in yeast
AssigneeSmithKline Beecham Biologicals s.a.
InventorsEric Jacobs, Apolonia Rutgers
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Abstract
The present invention provides a method for obtaining expression of a mixed hepatitis B surface antigen particle, e.g., a particle of mixed polypeptide composition, in yeast. Also disclosed is a method for obtaining multimeric structures presenting two or more polypeptides by expression in yeast as fusion proteins with the S antigen of HBsAg.
Claims19
A method for producing a hepatitis B surface antigen particle comprising coexpressing in a yeast cell a first gene encoding a first protein S and a second gene encoding a second protein X-S, wherein S is substantially the major surface protein of hepatitis B virus and wherein X is all or a portion of the preS1S2 polypeptide of hepatitis B virus optionally fused to another polypeptide.
Dependencies are inferred from the claim text.