Leptospiral major outer membrane protein LipL32
AssigneeUniversity of California
InventorDavid A. Haake
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Abstract
An antigenic preparation is provided containing an outer membrane protein associated with pathogenic strains of Leptospira. The protein has been designated “LipL32” for “lipoprotein from Leptospira” and because the isolated polypeptide migrates to a position corresponding to a molecular weight of 32 kD in a denaturing polyacrylamide gel. The invention provides polynucleotides encoding LipL32 and antibodies that bind the protein which are useful in the diagnosis of leptospirosis. In addition, LipL32 can be used immunologically as a vaccine for spirochete-associated pathologies.
Claims16
An isolated polynucleotide segment encoding an amino acid sequence as set forth in SEQ ID NO:2.
An isolated polynucleotide segment selected from the group consisting of:
- a) SEQ ID NO:1;
- b) SEQ ID NO:1, wherein each of the T residues is a U;
- and
- c) nucleic acid sequences fully complementary to SEQ ID NO:1.
A kit useful for the detection of a LipL32 nucleic acid, the kit comprising one or more containers comprising a first container containing a polynucleotide selected from the group consisting of:
- a) SEQ ID NO:1;
- b) SEQ ID NO:1, wherein each of the T residues is a U;
- and
- c) nucleic acid sequences fully complementary to SEQ ID NO:1.
An isolated polynucleotide segment encoding an amino acid sequence as set forth in SEQ ID NO:2 operably linked to a heterologous nucleic acid sequence.
Dependencies are inferred from the claim text.