Biopolymer arrays and their fabrication
AssigneeAgilent Technologies, Inc.
InventorMichel G. M. Perbost
Life of this patent
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Abstract
A method of fabricating an addressable array of biopolymers on a substrate using a biomonomer with a first linking group which must be activated for linking to a substrate bound moiety, and apparatus and computer program products for executing the method. The method includes forming on a region of the substrate carrying the substrate bound moiety, a solid activator composition. A biomonomer containing fluid composition is deposited on the region so that the solid activator activates the first linking group and the biomonomer links to the substrate bound moiety. The foregoing steps may be repeated, wherein a biomonomer deposited and linked to the substrate bound moiety in one cycle is the substrate bound moiety for the next cycle, so as to form the biopolymer.
Claims25
A method of fabricating an addressable array of biopolymers on a substrate using a biomonomer with a first linking group which must be activated for linking to a substrate bound moiety, comprising, at each of multiple different regions of the substrate:
- (a) forming on a region of the substrate carrying the substrate bound moiety, a solid activator composition;
- (b) depositing a biomonomer containing fluid composition on the region so that the solid activator activates the first linking group and the biomonomer links to the substrate bound moiety;
- and
- (c) repeating steps (a) and (b), wherein a biomonomer deposited and linked to the substrate bound moiety in one cycle is the substrate bound moiety for the next cycle, so as to form the biopolymer;
- so as to form the addressable array;
- wherein the biomonomer fluid composition deposited at a region covers an area greater than that covered by the solid activator composition at the same region.
A method of fabricating an addressable array of biopolymers on a substrate using biomonomers each with a first linking group which must be activated for linking to a substrate bound moiety, and a deposition system with a head having multiple pulse jets each of which can dispense droplets of a fluid onto a substrate, each jet including a chamber with an orifice, and including an ejector which, when activated, causes a droplet to be ejected from the orifice, the method comprising:
- (a) depositing onto a region of the substrate carrying the substrate bound moiety, a fluid comprising of a solid activator;
- (b) allowing fluid of the composition to evaporate to form the solid activator on the region;
- (c) then depositing from a pulse jet onto the region, a droplet of a biomonomer containing fluid composition so that the solid activator activates the first linking group and the biomonomer links to the substrate bound moiety;
- and
- (d) repeating steps (a) through (c) at each of multiple regions of the substrate, wherein at each region a biomonomer deposited and linked to the substrate bound moiety in one cycle is the substrate bound moiety for the next cycle, so as to form the addressable array of biopolymers;
- wherein a droplet of biomonomer fluid composition deposited at a region will cover an area greater than that covered by a preceding droplet of activator fluid composition at the same region.
Dependencies are inferred from the claim text.