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Assigneenovember Aktiengesellschaft Gesellschaft fur Molekulare Medizin
InventorWolf Bertling
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The invention relates to a method of detecting and identifying nucleic aicd (NA) molecules within a population (I) of NA molecules of identical, similar or different sequence, in which: (a) the single-stranded or duplex ribonucleic acid (RNA) molecules or single-stranded deoxyribonucleic aicd (DNA) molecules contained in a population (I) iare convereted into a population (II) of duplex DNA molecules; b) the population (II) of duplex DNA molecules is subjected to denaturation and subsequent renaturation, so that a mixture of homo- and heteroduplex DNA molecules is present; c) heteroduplexes formed in the population (II) where unpaired of incorrectly paired nucleotides are present are reacted chemically with a carbodiimide compound; and d) the resulting carboddimide reactions products are characterized and quantified, characterized in that a chromatographic purification method is carried out between each of the steps a to d.
A method of detecting and quantifying nucleic acid molecules within a population I of nucleic acid molecules of identical, similar or different sequence, comprising a) converting single-stranded or duplex ribonucleic acid (RNA) molecules or single-stranded deoxyribonucleic acid (DNA) molecules contained in population I into a population II of duplex DNA molecules, b) denaturating and subsequently renaturating the population II of duplex DNA molecules thereby forming a mixture of homo-duplex and heteroduplex DNA molecules, c) reacting chemically with a carbodiimide compound the heteroduplexes formed in the population II where unpaired or incorrectly paired nucleotides are present, d) detecting and quantifying the resulting carbodiimide reaction products, and performing a chromatographic purification method between each of steps a to d.
Dependencies are inferred from the claim text.