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US 6,306,591 B1Granted
Screening for the molecular defect causing spider lamb syndrome in sheep
AssigneeUtah State University
InventorsNoelle E. Cockett, Jonathan E. Beever
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AssigneeUtah State University
InventorsNoelle E. Cockett, Jonathan E. Beever
Estimating the term…
The present invention relates to the field of sheep genetics. Specifically, it relates to materials and methods used to detect a recessive gene causative for hereditary chondroplasia or “Spider Lamb Syndrome”. Disclosed are genetic markers for Spider Lamb Syndrome and methods for screening sheep to differentiate those that possess no, one, or two copies of the Spider lamb Syndrome defective gene. In addition, methods are described for identifying other markers associated with Spider Lamb Syndrome. The markers are based upon the presence or absence of certain polymorphisms in the ovine fibroblast growth factor receptor 3 gene. Preferably, the polymorphisms are detected as polymerase chain reaction-restriction fragment length polymorphisms or single strand conformational polymorphisms.
A method of identify a genetic marker for Spider Lamb Syndrome in a sheep, said method comprising:
A method of performing a nucleotide amplification reaction using a DNA sample from sheep and a pair of single stranded oligonucleotide primers for determining all or part of a nucleotide sequence of a Spider Lamb Syndrome gene, said primers being selected from a sequence set forth in SEQ ID No:1.
A method for identifying a mutant fibroblast growth factor receptor 3 gene sequence in a sheep suspected of comprising a mutant Spider Lamb Syndrome allele, said method comprising:
A method for screening a sheep to determine if said sheep carries the gene for Spider Lamb Syndrome, said method comprising:
An isolated nucleotide sequence having at most 138 nucleotides selected from the group of sequences consisting of the sequence set forth in SEQ ID NO:
A kit for detecting mutation in a fibroblast growth factor receptor 3 gene of a sheep comprising at least one oligonucleotide primer specific for a sheep fibroblast growth factor receptor 3 gene mutation, and instructions relating to detecting mutations in the fibroblast growth factor receptor 3 gene of the sheep.
A kit for assaying for the presence for a FGFR3 gene in a sheep by hybridization assay techniques, said kit comprising:
Dependencies are inferred from the claim text.