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US 6,306,590 B1Granted
Microfluidic matrix localization apparatus and methods
AssigneeCaliper Technologies Corp.
InventorsTammy Burd Mehta, Anne R. Kopf-Sill
Life of this patent
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Abstract
Multiphasic microfluidic apparatus for performing product fluid manipulation and separation in a single continuous unit are provided. Related methods, kits, and compositions are also provided.
Claims19
A method of performing a polymerase chain reaction in a microfluidic apparatus, said method comprising:
- providing a microfluidic device comprising at least two intersecting channels in fluid communication;
- filling at least one of said two intersecting channels with an electrophoretic phase selected from the group consisting of a sieving matrix and a molecular partition matrix, wherein the electrophoretic phase comprise at least two PCR reactants and a thermostable polymerase dispersed therein;
- performing the polymerase chain reaction in the presence of the electrophoretic phase to produce at least a first product.
A method of performing a polymerase chain reaction in a microfluidic apparatus, said method comprising:
- providing a microfluidic device comprising at least two intersecting channels in fluid communication;
- filling at least one of said two intersecting channels with a mixture comprising a sieving matrix, a thermostable polymerase and a plurality of PCR reaction components;
- performing the PCR in the presence of the sieving matrix to produce at least a first product.
Dependencies are inferred from the claim text.