Method for measuring enzyme reaction
AssigneeSeikagaku Corporation
InventorsHiroshi Tamura, Shigenori Tanaka
Life of this patent
Estimating the term…
Abstract
A method for measuring an enzyme reaction to determine an amount of a substance involved in the enzyme reaction, which comprises measuring a time course of a parameter of the enzyme reaction, measuring a time required for the parameter of the enzyme reaction to change from a first threshold value to a second threshold value, and correlating the measured time to an amount of the substance involved in the enzyme reaction.
Claims6
A method for measuring an enzyme reaction to determine an amount of a substance involved in the enzyme reaction wherein said substance is endotoxin, (1→3)-β-D-glucan, or peptidoglycan, and said enzyme reaction is a Limulus reaction or a phenol oxidase precursor cascade reaction, which method comprises the steps of:
- preselecting a parameter of the enzyme reaction, said parameter being indicative of a time course of the enzyme reaction,
- starting the enzyme reaction,
- measuring a time required for the parameter of the enzyme reaction to change from a first threshold value to a second threshold value, said first threshold value being set within 0.1 to 10% of a maximum change in the parameter of the enzyme reaction, and
- correlating the measured time to an amount of the substance involved in the enzyme reaction.
Dependencies are inferred from the claim text.