Back to results
US 6,306,576 B1Granted
Diagnostic methods for asthma
AssigneeThe Cleveland Clinic Foundation
InventorsStan Hazen, Weijia Wu, David Schmitt
Life of this patent
Estimating the term…
Loading patent…
Loading patent…
AssigneeThe Cleveland Clinic Foundation
InventorsStan Hazen, Weijia Wu, David Schmitt
Estimating the term…
Screening methods for asthma and analogous diseases in which activated eosinophils are found at the disease site are provided. The methods involve assaying for the presence of brominated tyrosine species in a bodily sample which has been obtained from a test subject. The brominated tyrosine species are either free in the sample or protein bound. In one embodiment, the assay involved measuring the amount of a brominated tyrosine species, particularly 3-bromotyrosine, 3,5-dibromotyrosine, or combinations thereof (referred to hereinafter collectively as the “diagnostic marker”) in a bodily sample from the test subject. In another embodiment for determining the prognosis of asthma in a test subject, the concentration or content of the diagnostic marker is determined in bodily samples taken from the test subject over successive time intervals. The concentrations are compared to determine the prognosis of the asthma. In another embodiment of the invention for monitoring the response of the test subject to treatment with an anti-asthmatic drug, the concentration or content of the diagnostic marker is measured in bodily samples obtained from the test subject before and after such treatment. The present invention also relates to a diagnostic kit and to a diagnostic reagent for diagnosing asthma and analogous diseases which are associated with activated eosinophils.
A method for diagnosing a disease associated with activated eosinophils in a subject comprising:
A method of diagnosing asthma in a subject suspected of having asthma comprising:
A diagnostic kit for diagnosing diseases in which activated eosinophils are present at the discase site, said kit comprising an antibody that specifically reacts with a brominated tyrosine species, wherein the brominated tyrosine species is contained within a peptide or protein.
A method of diagnosing an allergy in a subject suspected of having an allergy comprising:
A method of diagnosing a disease associated with inflammation and activated eosinophils in a subject comprising:
A diagnostic kit for diagnosing diseases in which activated eosinophils are present at the disease site, said kit comprising an antibody that specifically reacts with 3-bromotyrosine, or 3'5 di-bromotyrosine, or 3-bromotyrosine and a 3,5dibromotyrosine, wherein the bromotyrosine is either free or contained within a peptide or protein.
Dependencies are inferred from the claim text.