Method of preparing pharmaceutical compositions
AssigneePharmacia Corporation
InventorsMattias Andersson, Dorota Johansson, Percy Li, Björn Norrlind, Bengt Westgren
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Abstract
The invention relates to a method of preparing a composition of lipid particles comprising a bioactive protein, capable of being subjected to high shear forces without substantial loss of activity, and a lipid agent. The characterizing features of the method are the introduction of a protein preparation and a lipid agent to a homogenization station, whereupon the resulting fluid mixture of protein an lipid agent is subjected to high pressure homogenization. The so formed lipid particles are collected and if necessary further processed into a pharmaceutical formulation.
Claims41
A method of preparing a composition of lipid particles comprising a lipid agent and a protein, wherein said protein is an apolipoprotein, in hydrophobic interaction comprising:
- (i) introducing a protein preparation and a lipid agent, the protein capable of hydrophobic interaction with the lipid agent, to a homogenization station;
- (ii) subjecting resulting fluid mixture of protein and lipid agent to high pressure homogenization at a pressure from about 200 bar to about 2000 bar;
- and
- (iii) collecting the so formed composition of lipid particles, thereby yielding at least 75 percent lipid associated protein.
A method of preparing a composition of lipid particles comprising a lipid agent and a protein, wherein said protein is an apolipoprotein, in hydrophobic interaction comprising:
- (i) introducing protein preparation and a lipid agent, the protein capable of hydrophobic interaction with the lipid agent, to a homogenization station;
- (ii) subjecting resulting fluid mixture of protein and lipid agent to high pressure homogenization at a pressure from about 200 bar to about 2000 bar;
- and
- (iii) collecting the so formed composition of lipid particles, wherein the protein is not denatured in the preparation of the lipid particles.
Dependencies are inferred from the claim text.