Drug delivery system involving interaction between protein or polypeptide and hydrophobic biodegradable polymer
AssigneeUniversity of Kentucky Research Foundation
InventorPatrick P. Deluca
Life of this patent
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Abstract
A drug delivery system for controlled release of a protein or polypeptide comprising a hydrophobic biodegradable polymer and a protein or polypeptide. A physical interaction is present between the polymer and the protein or polypeptide, thus, allowing protection and controlled release of the protein or polypeptide in-vivo. The drug delivery system may be prepared by a polymer precipitation technique or a microsphere technique.
Claims15
A drug delivery system for controlled release of calcitonin, said delivery system comprising a biodegradable hydrophobic polymer selected from the group consisting of polyglycolic acid, polylactic acid, copolymers of glycolic acid and L- or D,L-lactic acid, and copolymers of glycolide and L- or D,L-lactide;
- and calcitonin, wherein calcitonin is adsorbed to the polymer and a hydrophobic bond is present between said polymer and calcitonin whereby protection and controlled release of calcitonin is achieved in vivo.
A process for preparing a drug delivery system for controlled release of calcitonin, said process comprising:
- (a) dissolving calcitonin and a hydrophobic biodegradable polymer selected from the group consisting of polyglycolic acid, polylactic acid, copolymers of glycolic acid and L- or D,L-lactic acid, and copolymers of glycolide and L- or D,L-lactide, in a first solvent and dispersing the solution in a second solvent to form microdroplets,
- (b) extracting the first solvent from the microdroplets, such that an interaction occurs at the aqueous polymer interface causing the calcitonin to adsorb to the polymer, and hardening the polymer such that calcitonin-loaded microspheres are formed.
A process for preparing a drug delivery system for controlled release of calcitonin, said process comprising:
- (a) dissolving calcitonin and a hydrophobic biodegradable polymer selected from the group consisting of polyglycolic acid, polylactic acid, copolymers of glycolic acid and L- or D,L-lactic acid, and copolymers of glycolide and L- or D,L-lactide, in a first solvent, and
- (b) adding a second solvent in which calcitonin is soluble, causing the polymer to precipitate and form microparticles to which calcitonin is adsorbed.
A process for preparing a drug delivery system for controlled release of calcitonin, said process comprising:
- a) dissolving calcitonin and a hydrophobic biodegradable polymer selected from the group consisting of polyglycolic acid, polylactic acid, copolymers of glycolic acid and L- or D,L-lactic acid, and copolymers of glycolide and L- or D,L-lactide, in a first solvent to form a first phase;
- b) dispersing said first phase in a continuous second solvent phase to obtain a suspension, and
- c) removing said first and second solvents from said suspension by freeze-drying to obtain microspheres containing the calcitonin and polymer.
A drug delivery system for controlled release of calcitonin, said delivery system comprising a biodegradable hydrophobic polymer selected from the group consistion of polyglycolie acid, polylactic acid, copolymers of glycolic acid and L- or D,L-lactic acid, and copolymers of glycolide and L- or D,L-lactide;
- and calcitonin, wherein calcitonin is adsorbed to the polymer and a hydrophobic bond is present between said polymer and calcitonin, and not a covalent bond or hydrogen bond as determined by differential scanning calorimetry, infrared spectroscopy, Fourier transform infrared spectroscopy, Raman spectroscopy and Fourier transform Raman spectroscopy, whereby protection and controlled release of calcitonin is achieved in vivo.
A process for preparing a drug delivery system for controlled release of calcitonin, said process comprising:
- (a) dissolving calcitonin and a hydrophobic biodegradable polymer selected from the group consisting of polyglycolic acid, polylactic acid, copolymers of glycolic acid and L- or D,L-lactic acid, and copolymers of glycolide and L- or D,L-lactide, in a first solvent and dispersing the solution in a second solvent to form microdroplets,
- (b) extracting the first solvent from the microdroplets, such that an interaction occurs at the aqueous polymer interface causing the calcitonin to adsorb to the polymer and form a hydrophobic bond, and hardening the polymer such that calcitonin-loaded microspheres are formed.
A process for preparing a drug delivery system for controlled release of calcitonin, said process comprising:
- (a) dissolving calcitonin and a hydrophobic biodegradable polymer selected from the group consisting of polyglycolic acid, polylactic acid, copolymers of glycolic acid and L- or D,L-lactic acid, and copolymers of glycolide and L- or D,L-lactide, in a first solvent, and
- (b) adding a second solvent in which calcitonin is soluble, causing the polymer to precipitate and form microparticles to which calcitonin is adsorbed, wherein a hydrophobic bond is present between said polymer and calcitonin.
A process for preparing a drug delivery system for controlled release of calcitonin, said process comprising:
- a) dissolving calcitonin and a hydrophobic biodegradable polymer selected from the group consisting of polyglycolic acid, polylactic acid, copolymers of glycolic acid and L- or D,L-lactic acid, and copolymers of glycolide and L- or D,L-lactide, in a first solvent to form a first phase;
- b) dispersing said first phase in a continuous second solvent phase to obtain a suspension, and
- c) removing said first and second solvents from said suspension by freeze-drying to obtain microspheres containing the calcitonin and polymer, wherein calcitonin is adsorbed to the polymer and a hydrophobic bond is present between said polymer and calcitonin.
Dependencies are inferred from the claim text.