Use of microparticles combined with submicron oil-in-water emulsions
AssigneeChiron Corporation
InventorsDerek O'Hagan, Gary Van Nest, Gary S. Ott, Manmohan Singh
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Abstract
Compositions are provided which include biodegradable microparticles with entrapped or adsorbed antigens, in combination with submicron oil-in-water emulsions. Also provided are methods of immunization which comprise administering to a vertebrate subject (a) a submicron oil-in-water emulsion, and (b) a therapeutically effective amount of a selected antigen entrapped in a microparticle.
Claims20
A method of inducing an immune response which comprises administering to a vertebrate subject (a) a submicron oil-in-water emulsion, wherein the submicron oil-in-water emulsion comprises 4-5% w/v squalene, 0.25-0.5% w/v polyoxvethvlene sorbitan monooleate, and 0.5% w/v sorbitan trioleate;
- and (b) a therapeutically effective amount of a selected antigen entrapped in, or adsorbed to, a biodegradable microparticle, wherein the microparticle is formed from a poly (α-hydroxy acid) selected from the group consisting of poly(L-lactide), poly(D,L-lactide) and poly(D,L-lactide-co-glvcolide), wherein administration of the submicron oil-in-water emulsion is either prior to, concurrent with, or subsequent to delivery of the microparticle.
A method of inducing an immune response which comprises administering to a vertebrate subject a composition comprising (a) a submicron oil-in-water emulsion comprising 4-5% w/v squalene, 0.25-0.5% w/v polyoxyethylene sorbitan monooleate, and 0.5% w/v sorbitan trioleate, and (b) a selected antigen entrapped in, or adsorbed to, a poly(D,L-lactide-co-glycolide) microparticle.
Dependencies are inferred from the claim text.