Compositions which inhibit apoptosis, methods of purifying the compositions and uses thereof
AssigneeLXR Biotechnology Inc.
InventorsIan Cyril Bathurst, John D. Bradley, L. David Tomei, Philip J. Barr
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Abstract
The present invention is to methods of obtaining plant-derived compositions that inhibit apoptosis, the compositions obtained thereby, compositions comprising the composition, and methods of use thereof.
Claims36
A method of obtaining a composition comprising a factor with anti-apoptotic activity wherein the method comprises the steps of
- a) delipidating a plant powder by extraction of the powder with a solution containing an organic solvent;
- b) separating the powder from the organic solvent;
- c) extracting the delipidated powder with an aqueous solution selected from the group consisting of (i) an aqueous solution comprising a water-miscible organic solvent at a concentration of up to 80% of the solution and (ii) phosphate buffered saline;
- and
- d) separating the aqueous solution from the delipidated powder to obtain an aqueous retentate composition comprising a factor with anti-apoptotic activity.
A method of obtaining a composition with anti-apoptotic activity, comprising the steps of:
- a) obtaining a powder of dried peas;
- b) delipidating the powder with an organic phase comprising 70% acetone, wherein the organic phase is in a volume approximately equal to the weight of the powder;
- c) separating the acetone from the delipidated powder;
- d) extracting the delipidated powder with an aqueous phase comprising ethanol and water, wherein the aqueous phase is in a volume approximately equal to the weight of the delipidated powder;
- e) separating the aqueous phase from the extracted plant powder to obtain an aqueous retentate;
- f) removing the ethanol from the aqueous retentate by filtration;
- and
- g) lyophilizing the product of step f) to obtain a composition with anti-apoptotic activity.
A method of obtaining a composition comprising a factor with anti-apoptotic activity wherein the method comprises the steps of:
- a) delipidating a plant powder by extraction of the powder with a solution containing an organic solvent;
- b) separating the powder from the organic solvent;
- c) extracting the delipidated powder with an aqueous solution;
- d) separating the aqueous solution from the delipidated powder to obtain an aqueous retentate composition comprising a factor with anti-apoptotic activity;
- and
- e) precipitating contaminants of the aqueous retentate composition.
A method of obtaining a composition comprising a factor with anti-apoptotic activity wherein the method comprises the steps of:
- a) delipidating a plant powder by extraction of the powder with a solution containing an organic solvent;
- b) separating the powder from the organic solvent;
- c) extracting the delipidated powder with an aqueous solution;
- d) subjecting the aqueous solution to size exclusion chromatography to obtain an aqueous retentate composition comprising a factor with anti-apoptotic activity;
- and
- e) precipitating contaminants of the aqueous retentate composite.
Dependencies are inferred from the claim text.