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AssigneeAquila Biopharmaceuticals, Inc.
InventorsCheryl Murphy, James Storey, Gerald A. Beltz, Richard T. Coughlin
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Nucleic acids encoding eleven different proteins of granulocytic erhlichia (GE), a tick-borne intracellular bacteria, have been isolated and sequenced completely. These DNAs were isolated as immunoreactive clones from a Lambda Zap II genomic library of GE DNA purified from infected HL60 cells. Three of the clones, E8, E80, and E46, contain open reading frames for four highly homologous proteins which appear to be part of a multigene family resembling the MSP-2 gene family of Anaplasma marginale. One clone, B3, contained a gene encoding the heat shock 70 protein. The other clones (W20, E74, and E82) contain open reading frames for proteins which have some homology to other bacterial proteins present in the nucleotide and protein databases. These and other GE antigens identified by immunoscreening of the genomic library are potentially useful as diagnostic reagents and vaccine candidates for GE.
A purified polypeptide comprising an amino acid sequence selected from the group of amino acid sequences set forth as SEQ ID NOS:
A purified polypeptide having at least 85% amino acid sequence identity to the amino acid sequence set forth as SEQ ID NO:
A purified polypeptide having at least 90% amino acid sequence identity to the amino acid sequence set forth as SEQ ID NO:
A purified polypeptide, comprising an immunologically reactive fragment of the amino acid sequence set forth in SEQ ID NO:
Dependencies are inferred from the claim text.