Methods for preparing polynucleotide transfection complexes
AssigneeValentis, Inc.
InventorsRobert Bridenbaugh, Warren Dang, Gary Koe
Life of this patent
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Abstract
Methods are provided for the preparation of transfection complexes of polynucleotides and polycations, especially cationic lipids, suitable for delivering polynucleotides to cells. In particular, methods are provided for preparing transfection complexes using a reduced-volume, dual-feed process. Complexes are formed upon the collision of two feed stream, containing polynucleotides and polycation, respectively, under conditions resulting in turbulent mixing conditions in minimal volume, and removal of transfection complexes under laminar flow conditions. Alternatively, the components are mixed in a static mixer. The process is easily scaleable and highly reproducible.
Claims8
A method of preparing a polynucleotide transfection complex, said method comprising:
- providing a first feed stream comprising a polynucleotide in solution and a second feed stream comprising a polycation in solution;
- mixing the first and second feed stream using a static mixer whereby polynucleotide transfection complexes are formed in solution;
- and
- removing the solution of polynucleotide transfection complexes.
A method of preparing a polynucleotide transfection complex comprising:
- providing a first feed stream comprising polynucleotides in solution and a second feed stream comprising cationic liposomes in solution;
- mixing the first and second feed stream using a static mixer whereby polynucleotide transfection complexes are formed in solution;
- and
- removing the solution of polynucleotide transfection complexes.
Dependencies are inferred from the claim text.