Back to results
US 6,303,344 B1Granted
Methods and compositions for polypeptide engineering
AssigneeMaxygen, Inc.
InventorsPhillip A. Patten, Willem P.C. Stemmer
Life of this patent
Estimating the term…
Abstract
Methods are provided for the evolution of proteins of industrial and pharmaceutical interest, including methods for effecting recombination and selection. Compositions produced by these methods are also disclosed.
Claims12
A method for selecting or screening an evolved DNA substrate molecule encoding an interferon alpha, comprising:
- (a) providing a library of mutant alpha interferon genes, the library comprising an expression vector wherein the alpha interferon genes are expressed under the control of an inducible promoter;
- (b) transfecting host cells with the library of (a);
- (c) contacting the product of (b) with a virus;
- (d) recovering DNA encoding a mutant alpha interferon from host cells surviving step (c);
- and
- (e) recovering an evolved interferon gene from the product of (d).
3 more elements
Dependencies are inferred from the claim text.