Pancreas-derived plasminogen activator inhibitor
AssigneeHuman Genome Sciences, Inc.
InventorsJian Ni, Reiner L. Gentz, Steven M. Ruben, Y. Eric Shi
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Abstract
The present invention relates to a novel member of the plasminogen activator inhibitor protein family. In particular, isolated nucleic acid molecules are provided encoding the pancreas-derived plasminogen activator inhibitor protein. Pancreas-derived plasminogen activator inhibitor polypeptides are also provided as are vectors, host cells and recombinant methods for producing the same. The invention further relates to methods for treating physiologic and pathologic disease conditions, including breast cancer, and diagnostic methods for detecting pathologic disorders.
Claims79
An isolated polynucleotide, comprising a nucleic acid encoding an amino acid sequence at least 95% identical to a reference amino acid sequence selected from the group consisting of:
- (a) amino acids −14 to 378 of SEQ ID NO:2;
- (b) amino acids −13 to 378 of SEQ ID NO:2;
- and
- (c) amino acids 1 to 378 of SEQ ID NO:2;
- wherein said polynucleotide encodes a polypeptide which binds to an antibody that specifically binds a polypeptide consisting of amino acids 1 to 378 of SEQ ID NO:2.
An isolated polynucleotide, comprising a nucleic acid encoding an amino acid sequence at least 95% identical to a reference amino acid sequence selected from the group consisting of:
- (a) the full length polypeptide as encoded by the cDNA clone contained in ATCC Deposit No. 97656;
- and
- (b) the mature polypeptide as encoded by the cDNA clone contained in ATCC Deposit No. 97656;
- wherein said polynucleotide encodes a polypeptide which binds to an antibody that specifically binds a polypeptide consisting of the mature amino acid sequence as encoded by the cDNA clone contained in ATCC Deposit No. 97656.
An isolated polynucleotide, comprising a nucleic acid encoding an amino acid sequence at least 95% identical to a reference amino acid sequence selected from the group consisting of:
- (a) amino acids −18 to 387 of SEQ ID NO:13;
- (b) amino acids −17 to 387 of SEQ ID NO:13;
- and
- (c) amino acids 1 to 387 of SEQ ID NO:13;
- wherein said polynucleotide encodes a polypeptide which binds to an antibody that specifically binds a polypeptide consisting of amino acids 1 to 378 of SEQ ID NO:13.
An isolated polynucleotide comprising a nucleic acid encoding amino acids 9 to 76 of SEQ ID NO:2.
An isolated polynucleotide comprising a first nucleic acid which hybridizes (i) at 42° C. in a solution consisting of 50% formamide, 5×SSC, 50 mM sodium phosphate, 5×Denhardt's solution, 10% dextran sulfate, and 20 μg/ml denatured, sheared salmon sperm DNA;
- (ii) followed by washing in a solution consisting of 0.1×SSC at 65° C.;
- to a second nucleic acid having the nucleotide sequence of the coding region of SEQ ID NO:1 or SEQ ID NO:12 or the complement thereof;
- wherein said first nucleic acid encodes a polypeptide which inhibits a plasminogen activator.
Dependencies are inferred from the claim text.