US 6,300,142 B1Granted

Device and apparatus for conducting an assay

AssigneeProvalis Diagnostics Limited

InventorsDavid Andrewes, John Worthington Attridge, David Griffiths, John Phillip Vessey, John Anthony Odell, Felix Fernando, Anthony Stevenson, Mark Gresswell, John Curtis, David Alan Percival

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Abstract

A apparatus for assaying glycated proteins and other analytes in biological samples such as blood, in which a sample is presented to the apparatus, includes an inlet port between moveable between first and second inlets, such that the inlet can be brought into liquid communication with each inlet in turn. The inlet port accommodates a filter or binder. The apparatus also includes a microprocessor operable via a key pad, at least one light emitter and at least one light detector, a display and driver, and an A to D converter, and is operatively connected to a power source.

Claims20

Dependent claims

An apparatus for use in an assay in which a sample is separated into component fractions for presentation to an instrument, said apparatus comprising:

a carousel, including,
(1) a base portion having
(a) a first inlet and a first component fraction collection chamber defined therein, said first inlet being in fluid communication with said first component fraction collection chamber, and
(b) a second inlet and a second component fraction collection chamber defined by said base portion, said second inlet being in fluid communication with said second component fraction collection chamber, wherein said first and second component collection fraction chambers are not in fluid communication with each other, and
(2) a top portion;
and
a funnel portion including an outlet port, said funnel portion being adapted to receive a filter means or a binder means,
wherein said carousel is rotatably mounted about said funnel portion such that when said carousel is rotated, said outlet port is brought into selective liquid communication with each first and second inlet in turn.

5 more elements

A method for assaying a sample with an apparatus having

(A) a carousel which includes
(1) a base portion having
(a) a first inlet and a first component fraction collection chamber defined therein, said first inlet being in fluid communication with said first component fraction collection chamber, and
(b) a second inlet and a second component fraction collection chamber defined by said base portion, said second inlet being in fluid communication with said second component fraction collection chamber, and
(2) a top portion having
(B) a funnel portion including an outlet port adapted to receive a filter means or a binder means, said carousel being rotatably mounted about said funnel portion such that as said carousel is rotated, said outlet port is moveable relative to each of said first and second inlets and is brought into selective liquid communication with each first and second inlet in turn, said method comprising the steps of:
collecting a first component fraction of the sample into the first component fraction collection chamber,
rotating said carousel about said funnel portion;
collecting a second component fraction of the sample into the second component fraction collection chamber;
and
assaying the first and second component fractions to determine the presence or absence of one or more analytes in said component fractions.

8 more elements

Dependencies are inferred from the claim text.

Facts

Application: 09/555,472Filed 26 May 2000
Priority: GB 972534828 Nov 1997
Granted: 9 Oct 2001
Term ends: Not estimatedThe source records don’t support an estimate.

Classification20 codes

B01L 3/502
Containers for the purpose of retaining a material to be analysed, e.g. test tubes: with fluid transport, e.g. in multi-compartment structures
B01L 2200/16
Solutions for specific problems relating to chemical or physical laboratory apparatus: reagents, handling or storing thereof
B01L 2300/045
Connecting closures to device or container: whereby the whole cover is slidable
B01L 2300/046
Closures and closing means: function or devices integrated in the closure