Transgenic non-human animals for producing heterologous antibodies
AssigneeGenPharm International, Inc.
InventorsNils Lonberg, Robert M. Kay
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Abstract
The invention relates to transgenic non-human animals capable of producing heterologous antibodies and methods for producing human sequence antibodies which bind to human antigens with substantial affinity.
Claims4
A hybridoma comprising a B cell obtained from a transgenic mouse having a genome comprising a human heavy chain transgene and a human light chain transgene, said B cell fused to an immortalized cell suitable to generate a hybridoma, wherein said hybridoma produces a detectable amount of an immunoglobulin that specifically binds GROα.
A hybridoma 2C6 having ATCC accession number PTA-2017 wherein the hybridoma produces a detectable amount of an immunoglobulin that specifically binds interleukin-8.
A composition comprising a substantially pure immunoglobulin, wherein said immunoglobulin specifically binds GROα, and wherein said immunoglobulin consists of:
- a human sequence light chain composed of (1) a light chain variable region having a polypeptide sequence which is substantially identical to a polypeptide sequence encoded by a human VL gene segment and a human JL segment, and (2) a light chain constant region having a polypeptide sequence which is substantially identical to a polypeptide sequence encoded by a CL gene segment;
- and
- a human sequence heavy chain composed of (1) a heavy chain variable region having a polypeptide sequence which is substantially identical to a polypeptide sequence encoded by a human VH gene segment, optionally a D region, and a human JH segment, and (2) a constant region having a polypeptide sequence which is substantially identical to a polypeptide sequence encoded by a human CH gene segment.
Dependencies are inferred from the claim text.