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AssigneeAntibioticos S.p.A.
InventorsJose Luis Barredo Fuente, Marta Rodriguez Saiz, Alfonso J. Collados De La Vieja, Migeul Angel Moreno Valle, Francisco Salto Maldonado, Bruno Diez Garcia
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The invention relates to promoters of the genes glutamate dehydrogenase, β-acetylhexosaminidase and γ-actin and their use in systems of expression, secretion and anti-sense of filamentary fungi. The invention also relates to the use of the promoters of the genes which code: (I) glutamate dehydrogenase NADP depending (EC.1.4.1.4) of Penicillium chrysogenum, (II) γ-N-actylhexosaminidase (EC.3.2.1.52) of Penicillium chrysogenum and (III) γ-actin of Penicillium chrysogenum and Acrimonium chrysogenum, which can be used for the construction of potent vectors of expression and secretion useful both for P. chrysogenum and for A. chrysogenum and related species. These promoters can also be used for blocking the genic expression through anti-sense construction. Under the control of the above mentioned promoters, it is possible to conduct the expression of other genes in filamentary fungi, thereby increasing the production of antibiotics and/or proteins inherent to the same.
An isolated DNA comprising the promoter sequence of the gdh gene of P. chrysogenum or a DNA fragment thereof that is capable of directing expression of DNA downstream of said fragment in P. chrysogenum.
An isolated DNA encoding a glutamate dehydrogenase enzyme of P. chrysogenum.
An isolated DNA comprising SEQ ID NO:1 or a DNA fragment thereof that encodes a glutamate dehydrogenase enzyme.
An isolated peptide comprising the amino acid sequence of SEQ ID NO:5.
Dependencies are inferred from the claim text.