Method for in vitro production of bone
AssigneeIsoTis N.V.
InventorsClemens Antoni Van Blitterswijk, Joost Dick De Bruijn, Yvonne Pearl Bovell
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Abstract
The invention relates to a method for in vitro production of bone tissue, comprising the steps of: (a) applying undifferentiated mammalian cells, in particular autologous marrow cells, on a substrate; (b) directly contacting said cells with a culture medium for a sufficient time to produce a continuous matrix; (c) removing the substrate with the matrix from the culture medium. The produced matrix can be used for joint prostheses, maxillofacial implants, special surgery devices, or bone fillers. The contacted culture medium can also be used for the production of active factors such as growth factors.
Claims17
A method for production of bone tissue, comprising the steps of:
- (a) applying undifferentiated mammalian cells on an implant substrate formed from a material selected from the group consisting of metal, calcium phosphate, and polymer, wherein the material is sufficient to allow fixation of the implant substrate at a site of implantation;
- (b) directly contacting the undifferentiated cells with a culture medium for a sufficient time to differentiate the undifferentiated cells and form osteoblasts and wherein said osteoblasts produce an extracellular bone tissue matrix;
- (c) removing the substrate with the bone tissue matrix from the culture medium.
A product formed by a method for production of bone tissue, comprising the steps of:
- (a) applying undifferentiated mammalian cells on an implant substrate formed from a material selected from the group consisting of metal, calcium phosphate, and polymer, wherein the material is sufficient to allow fixation of the implant substrate at a site of implantation;
- (b) directly contacting the undifferentiated cells with a culture medium for a sufficient time to differentiate the undifferentiated cells and form osteoblasts and wherein the osteoblasts produce an extracellular bone tissue matrix;
- (c) removing the substrate with the bone tissue matrix from the culture medium.
Dependencies are inferred from the claim text.