# US 6,297,426 B1 · Methods of mediating female fertility in plants > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 6,297,426 B1 - Document ID: US6297426B1 - Status: Granted - Kind: B1 - Application number: 09/059,958 - Filed: 1998-04-14 - Granted: 2001-10-02 - Earliest claimed: 1990-06-12 - Assignees: Pioneer Hi-Bred International, Inc. - Inventors: Marc C. Albertsen; Larry R. Beach; Gary A. Huffman; John Howard - Web page: https://patentgrep.com/patent/US6297426B1 ## CPC - C12N 15/8289: {Male sterility} - C07K 14/415: from plants - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) ## Abstract A controllably constitutively female sterile plant is provided. A gene critical to female fertility is identified and the native gene inactivated. The gene is cloned, linked to an inducible promoter and inserted into the plant. The result is a plant that is constitutively female sterile with inducible female fertility. ## Claims ### Claim 1 A method for providing heritable, externally controllable female fertility in a plant, comprising the steps of: a) cloning a gene which codes for a gene product on which female fertility in the plant is dependent; b) linking the cloned gene in an expression sequence with an inducible promoter responsive to external control; c) rendering inoperative the gene from the native nuclear genome of the plant, which codes for the gene product; and d) inserting the expression sequence into the nuclear genome of the plant such that the plant is controllably female fertile. ### Claim 2 A method of reproducing a plant having heritable externally controllable female fertility resulting from rendering inoperative a gene which codes for a gene product on which female fertility is dependent, and insertion of a gene which codes for the same gene product, but which is linked in an expression sequence with an inducible promoter responsive to external control, comprising the steps of: a) planting seed of the plant to provide growing, female sterile plants; b) inducing conversion of the growing plants to female fertile form by growing the plants under conditions which induce the promoter to express the gene, thereby producing the gene product on which female fertility is dependent; c) open-pollinating the growing plants in isolation to produce seed; and d) harvesting the seed. ### Claim 3 A method of producing hybrid seed, comprising the steps of: a) planting, in cross pollinating juxtaposition, a first seed from a selected male fertile male parent line which is female sterile having female sterility resulting from replacement of a gene which codes for a gene product on which female fertility is dependent with a gene which codes for the same gene product linked in an expression sequence with an inducible promoter responsive to external control, and a second seed from a selected female parent line which is male sterile; b) growing the seed to mature plants under conditions which do not induce expression of the gene; c) cross pollinating the male-sterile female plant with pollen from the male-fertile male plant; and d) harvesting hybrid seed from the male-sterile female plant. ### Claim 4 A controllably female sterile plant comprising: a plant having a gene coding for a gene product on which female sterility is dependent, said gene linked to an inducible promoter and the gene from the native nuclear genome of the plant which codes for said gene product rendered inoperative. ### Claim 5 (depends on 4) Plant cells of the plant of claim 4. ### Claim 6 (depends on 4) Plant parts of the plant of claim 4. ### Claim 7 Hybrid seed produced by: a) planting, in cross pollinating juxtaposition, a first seed from a selected male fertile male parent line which is female sterile, having female sterility resulting from rendering inoperative a gene which codes for a gene product on which female fertility is dependent and insertion of a gene which codes for the same gene product linked in an expression sequence with an inducible promoter responsive to external control and a second seed from a selected female parent having male sterility, b) growing the seed to mature plants under conditions which do not induce expression of the gene; c) cross pollinating the male sterile female plant with pollen from the male-fertile male plant; and d) harvesting hybrid seed from the male-sterile female plant.