# US 6,297,364 B1 · Isolated nucleic acid molecule encoding cancer associated antigen, the antigen itself, and uses thereof

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- Number: US 6,297,364 B1
- Document ID: US6297364B1
- Status: Granted
- Kind: B1
- Application number: 09/061,709
- Filed: 1998-04-17
- Granted: 2001-10-02
- Earliest claimed: 1998-04-17
- Assignees: Ludwig Institute for Cancer Research; Cornell Research Foundation, Inc.; Memorial Sloan Kettering Cancer Center
- Inventors: Yao-Tseng Chen; Ali Gure; Solam Tsang; Elisabeth Stockert; Elke Jäger; Knuth Alexander; Lloyd J. Old
- Web page: https://patentgrep.com/patent/US6297364B1

## CPC

- C07K 14/4748: {Tumour specific antigens; Tumour rejection antigen precursors [TRAP], e.g. MAGE}
- A61P 35/00: Antineoplastic agents
- A61K 2039/505: {comprising antibodies}
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)

## Abstract

The invention relates to the isolation of a nucleic acid molecule which encodes a cancer associated antigen. Also a part of the invention is the antigen itself, and the uses of the nucleic acid molecule and the antigen, and peptides derived from it.

## Claims

### Claim 1

Isolated nucleic acid molecule which encodes a cancer associated antigen, whose amino acid sequence is identical to the amino sequence encoded by nucleotides 287 to 3715 of SEQ ID NO: 1.

### Claim 2 (depends on 1)

The isolated nucleic acid molecule of claim 1, consisting of nucleotides 287-3715 of SEQ ID NO: 1.

### Claim 3 (depends on 1)

The isolated nucleic acid molecule of claim 1, consisting of anywhere from nucleotide 1 through nucleotide 4265 of SEQ ID NO: 1, with the proviso that said isolated nucleic acid molecule contains at least nucleotides 287-3715 of SEQ ID NO: 1.

### Claim 4 (depends on 1)

Expression vector comprising the isolated nucleic acid molecule of claim 1, operably linked to a promoter.

### Claim 5 (depends on 3)

Expression vector comprising the isolated nucleic acid molecule of claim 3, operably linked to a promoter.

### Claim 6 (depends on 4)

Eukaryotic cell line or prokaryotic cell strain, transformed or transfected with the expression vector of claim 4.

### Claim 7 (depends on 5)

Eukaryotic cell line or prokaryotic cell strain, transformed or transfected with the expression vector of claim 5.

### Claim 8 (depends on 1)

Eukaryotic cell line or prokaryote cell strain, transformed or transfected with the isolated nucleic acid molecule of claim 1.

### Claim 9 (depends on 8)

The eukaryotic cell line of claim 8, wherein said cell line has been rendered non-proliferative.

### Claim 10 (depends on 8)

The eukaryotic cell line of claim 8, wherein said cell line is a fibroblast cell line.

### Claim 11 (depends on 1)

A viral expression vector which comprises a nucleotide sequence of a virus and the isolated nucleic acid molecule of claim 1.

### Claim 12 (depends on 11)

The viral expression vector of claim 11, wherein said virus is adenovirus or vaccinia virus.

### Claim 13 (depends on 11)

The viral expression vector of claim 11, wherein said virus is adenovirus.

### Claim 14 (depends on 11)

The viral expression vector of claim 11, wherein said virus is vaccinia virus.