# US 6,297,360 B1 · Amphipathic protein-1

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- Number: US 6,297,360 B1
- Document ID: US6297360B1
- Status: Granted
- Kind: B1
- Application number: 09/390,598
- Filed: 1999-09-03
- Granted: 2001-10-02
- Earliest claimed: 1998-03-27
- Assignees: Academia Sinica
- Inventors: Teng-yung Feng; Hae-jan Lin
- Web page: https://patentgrep.com/patent/US6297360B1

## CPC

- C12N 15/8281: {for bacterial resistance}
- C07K 14/245: Escherichia (G)
- C07K 14/415: from plants
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

This invention relates to amphipathic protein-1 polypeptides that can protect plants from tissue damage caused by the hypersensitive response, which is often elicited by bacterial infection in higher plants.

## Claims

### Claim 1

A substantially pure amphipathic polypeptide encoded by a nucleic acid hybridizing to the compliment of a reference nucleic acid consisting of SEQ ID NO:1, wherein the hybridization is performed in the presence of 0.2× SSC and 0.1% (w/v) sodium dodecylsulfate and at 68° C.

### Claim 2 (depends on 1)

The polypeptide of claim 1, wherein the polypeptide decreases the growth of a bacterium in a plant.

### Claim 3 (depends on 1)

The polypeptide of claim 1, wherein the polypeptide is derived from a cotton, pepper, tobacco, or tomato plant.

### Claim 4 (depends on 1)

The polypeptide of claim 1, wherein the polypeptide comprises SEQ ID NO:2.