Back to results
US 6,297,038 B1Granted
Amylase variants
AssigneeNovozymes A/S
InventorsHenrik Bisgård-Frantzen, Allan Svendsen, Torben Vedel Borchert
Life of this patent
Estimating the term…
Loading patent…
Loading patent…
AssigneeNovozymes A/S
InventorsHenrik Bisgård-Frantzen, Allan Svendsen, Torben Vedel Borchert
Estimating the term…
The present invention relates to variants of a parent α-amylase, which parent α-amylase (i) has an amino acid sequence selected from the amino acid sequences shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, and SEQ ID No. 7, respectively; or (ii) displays at least 80% homology with one or more of these amino acid sequences; and/or displays immunological cross-reactivity with an antibody raised against an α-amylase having one of these amino acid sequences; and/or is encoded by a DNA sequence which hybridizes with the same probe as a DNA sequence encoding an a-amylase having one of these amino acid sequences; in which variant: (a) at least one amino acid residue of the parent α-amylase has been deleted; and/or (b) at least one amino acid residue of the parent α-amylase has been replaced by a different amino acid residue; and/or (c) at least one amino acid residue has been inserted relative to the parent α-amylase; the variant having α-amylase activity and exhibiting at least one of the following properties relative to the parent α-amylase: increased thermostability; increased stability towards oxidation; and reduced Ca2+ dependency; with the proviso that the amino acid sequence of the variant is not identical to any of the amino acid sequences shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No.3 and SEQ ID No. 7, respectively.
A variant of a parent alpha-amylase enzyme having an increased thermostability or a decreased isoelectric point as compared to said parent alpha-amylase, wherein said parent alpha-amylase has an amino acid sequence having at least 80% homology to SEQ ID NO:1, and wherein said variant comprises a modification of said parent alpha-amylase corresponding to a position selected from the group consisting of R124, K185, V206, L217, Y243, L351, Q391, K444, Q86, Q346, Q391 and N457 in the amino acid sequence shown in SEQ ID NO:1.
A variant of a parent alpha-amylase enzyme having an increased thermostability or a decreased isoelectric point as compared to said parent alpha-amylase, wherein said parent alpha-amylase has an amino acid sequence having at least 80% homology to SEQ ID NO:2, and wherein said variant comprises a modification of said parent alpha-amylase corresponding to a position selected from the group consisting of R124, K185, V206, L217, Y243, L351, Q391, K444, Q86, Q346, Q391 and N457 in the amino acid sequence shown in SEQ ID NO:1.
A variant of a parent alpha-amylase enzyme having an increased thermostability or a decreased isoelectric point as compared to said parent alpha-amylase, wherein said parent alpha-amylase has an amino acid sequence having at least 80% homology to SEQ ID NO:3, and wherein said variant comprises a modification of said parent alpha-amylase corresponding to a position selected from the group consisting of R124, K185, V206, L217, Y243, L351, Q391, K444, Q86, Q346, Q391 and N457 in the amino acid sequence shown in SEQ ID NO:1.
A variant of a parent alpha-amylase enzyme having an increased thermostability or a decreased isoelectric point as compared to said parent alpha-amylase, wherein said parent alpha-amylase has an amino acid sequence having at least 80% homology to SEQ ID NO:7, and wherein said variant comprises a modification of said parent alpha-amylase corresponding to a position selected from the group consisting of R124, K185, V206, L217, Y243, L351, Q391, K444, Q86, Q346, Q391 and N457 in the amino acid sequence shown in SEQ ID NO:1.
A variant of a parent alpha-amylase enzyme having an increased stability towards oxidation as compared to said parent alpha-amylase, wherein said parent alpha-amylase has an amino acid sequence having at least an 80% homology to SEQ ID No. 1, and wherein said variant comprises (a) an amino acid substitution of said parent alpha-amylase at a position corresponding to M202 with an amino acid selected from the group consisting of A, R, N, D, Q, E, G, H, I, L, K, F, P, S, T, W, Y, or V and (b) a deletion at positions 183 and 184, wherein the amino acid sequence shown in SEQ ID NO:2 is used for numbering.
A variant of a parent alpha-amylase enzyme having an increased stability towards oxidation as compared to said parent alpha-amylase, wherein said parent alpha-amylase has an amino acid sequence having at least an 80% homology to SEQ ID No. 2, and wherein said variant comprises (a) an amino acid substitution of said parent alpha-amylase at a position corresponding to M202 with an amino acid selected from the group consisting of A, R, N, D, Q, E, G, H, I, L, K, F, P, S, T, W, Y, or V and (b) a deletion at positions 183 and 184, wherein the amino acid sequence shown in SEQ ID NO:2 is used for numbering.
A variant of a parent alpha-amylase enzyme having an increased stability towards oxidation as compared to said parent alpha-amylase, wherein said parent alpha-amylase has an amino acid sequence having at least an 80% homology to SEQ ID No. 3, and wherein said variant comprises (a) an amino acid substitution of said parent alpha-amylase at a position corresponding to M202 with an amino acid selected from the group consisting of A, R, N, D, Q, E, G, H, I, L, K, F, P, S, T, W, Y, or V and (b) a deletion at positions 183 and 184, wherein the amino acid sequence shown in SEQ ID NO:2 is used for numbering.
A variant of a parent alpha-amylase enzyme having an increased stability towards oxidation as compared to said parent alpha-amylase, wherein said parent alpha-amylase has an amino acid sequence having at least an 80% homology to SEQ ID No. 7, and wherein said variant comprises (a) an amino acid substitution of said parent alpha-amylase at a position corresponding to M202 with an amino acid selected from the group consisting of A, R, N, D, Q, E, G, H, I, L, K, F, P, S, T, W, Y, or V and (b) a deletion at positions 183 and 184, wherein the amino acid sequence shown in SEQ ID NO:2 is used for numbering.
A variant of a parent alpha-amylase enzyme having a reduced calcium dependency as compared said parent alpha-amylase, wherein said parent alpha-amylase has an amino acid sequence having at least an 80% homology to SEQ ID No. 1, and wherein said variant comprises:
A variant of a parent alpha-amylase enzyme having a reduced calcium dependency as compared said parent alpha-amylase, wherein said parent alpha-amylase has an amino acid sequence having at least an 80% homology to SEQ ID No. 2, and wherein said variant comprises:
A variant of a parent alpha-amylase enzyme having a reduced calcium dependency as compared said parent alpha-amylase, wherein said parent alpha-amylase has an amino acid sequence having at least an 80% homology to SEQ ID No. 7, and wherein said variant comprises:
A variant of a parent alpha-amylase enzyme, wherein said parent enzyme has an amino acid sequence which has at least 80% homology to SEQ ID NO:1, and wherein said variant comprises a deletion of an amino acid equivalent to T183 or G184 of the amino acid sequence shown in SEQ ID NO:1.
A variant of a parent alpha-amylase enzyme, wherein said parent enzyme has an amino acid sequence which has at least 80% homology to SEQ ID NO:2, and wherein said variant comprises a deletion of an amino acid equivalent to T183 or G184 of the amino acid sequence shown in SEQ ID NO:1.
A variant of a parent alpha-amylase enzyme, wherein said parent enzyme has an amino acid sequence which has at least 80% homology to SEQ ID NO:3, and wherein said variant comprises a deletion of an amino acid equivalent to T183 or G184 of the amino acid sequence shown in SEQ ID NO:1.
A variant of a parent alpha-amylase enzyme, wherein said parent enzyme has an amino acid sequence which has at least 80% homology to SEQ ID NO:7, and wherein said variant comprises a deletion of an amino acid equivalent to T183 or G184 of the amino acid sequence shown in SEQ ID NO:1.
A variant of a parent alpha-amylase enzyme, said parent having at least a 90% homology to the amino acid sequence depicted in SEQ ID NO:1, wherein said variant comprises one of the following mutations of said parent alpha-amylase corresponding to the mutations selected from the group consisting of:
A variant of a parent alpha-amylase enzyme, said parent having at least a 90% homology to the amino acid sequence depicted in SEQ ID NO:2, wherein said variant comprises one of the following mutations of said parent alpha-amylase equivalent to the mutations selected from the group consisting of:
A variant of a parent alpha-amylase enzyme, said parent having at least a 90% homology to the amino acid sequence depicted in SEQ ID NO:7, wherein said variant comprises one of the following mutations of said parent alpha-amylase equivalent to the mutations selected from the group consisting of:
Dependencies are inferred from the claim text.