Parallel high-performance liquid chromatography with serial injection
AssigneeSymyx Technologies, Inc.
InventorPetro Miroslav
Life of this patent
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Abstract
High-performance liquid chromatography (HPLC) methods and systems are disclosed that combine sequential, serial injection of a plurality of samples into mobile-phases supplied in parallel to two or more chromatographic columns, such that staggered, parallel separation of the plurality of samples is effected. Because injection of samples is relatively fast as compared to separation, substantial efficiencies are gained with respect to overall sample throughput. The disclosed HPLC methods and systems are preferably applied in connection with combinatorial chemistry, combinatorial material science and more particularly, combinatorial synthesis and screening of polymeric materials
Claims30
A method for characterizing a plurality of non-biological polymer samples with a liquid chromatography system, the method comprising
- continuously supplying a mobile phase in parallel through each of first and second chromatographic columns of a liquid chromatography system,
- serially loading first and second samples into a common injector,
- serially and distributively injecting the first and second samples into the continuously supplied mobile phase of the first and second chromatographic columns, respectively,
- separating at least one sample component of the injected first and second samples from other sample components thereof substantially simultaneously in the respective chromatographic columns, and
- detecting a property of at least one of the separated sample components of the first and second samples.
A method for characterizing components of a combinatorial library of non-biological polymer samples with a liquid chromatography system, the method comprising
- providing a library comprising ten or more different non-biological polymer samples, the non-biological polymer samples being polymerization product mixtures resulting from polymerization reactions that are varied with respect to reaction conditions, reactants, catalysts, catalyst precursors, initiators, additives or the relative amounts thereof,
- continuously supplying a mobile phase in parallel through four or more chromatographic columns of a liquid chromatography system,
- serially loading the ten or more non-biological polymer samples into a common injector,
- serially and distributively injecting the ten or more different non-biological polymer samples into the mobile phase of the four or more chromatographic columns,
- separating at least one sample component of four or more of the injected samples from other sample components thereof substantially simultaneously in the respective four or more chromatographic columns, and
- detecting a property of at least one of the separated sample components of the four or more samples.
A method for characterizing components of a plurality of samples with a liquid chromatography system, the method comprising
- pumping a mobile phase in parallel through four or more chromatographic columns of a liquid chromatography system, the mobile phase being supplied continuously to each of the four or more chromatographic columns through a flow path comprising one or more flow restrictors,
- serially loading ten or more different samples into an injector,
- serially and distributively injecting the ten or more samples through a multi-port switching valve into the mobile phase of the four or more chromatographic columns,
- controlling the multi-port switching valve such that the ten or more samples are distributively injected into the mobile phase of a first, a second, a third and a fourth of the four or more chromatographic columns,
- separating at least one sample component of the injected ten or more samples from other sample components thereof in the respective chromatographic columns, and
- detecting a property of at least one of the separated sample components of the ten or more samples.
A method for evaluating interactions between a plurality of liquid samples and one or more solid or supported materials, the method comprising
- supplying a mobile phase in parallel through four or more columns comprising solid or supported materials, the mobile phase being supplied continuously to each of the four or more columns through a flow path comprising one or more flow restrictors,
- serially loading ten or more different liquid samples into an injector,
- serially and distributively injecting the ten or more samples through a multi-port switching valve into the mobile phase of the four or more columns,
- controlling the multi-port switching valve such that the ten or more samples are distributively injected into the mobile phase of a first, a second, a third and a fourth of the four or more columns, and
- evaluating the interactions between one or more components of the injected ten or more samples and the solid or supported materials.
Dependencies are inferred from the claim text.