# US 6,294,660 B1 · Nucleic acids encoding B7-1 and B7-2 molecules

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- Number: US 6,294,660 B1
- Document ID: US6294660B1
- Status: Granted
- Kind: B1
- Application number: 08/702,525
- Filed: 1997-02-07
- Granted: 2001-09-25
- Earliest claimed: 1994-03-02
- Assignees: Dana-Farber Cancer Institute Brigham; Women's Hospital
- Inventors: Arlene H. Sharpe; Francescopaolo Borriello; Gordon J. Freeman; Lee M. Nadler
- Web page: https://patentgrep.com/patent/US6294660B1

## CPC

- C07K 14/70532: {B7 molecules, e.g. CD80, CD86}
- A01K 2217/05: Animals comprising random inserted nucleic acids (transgenic)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- A01K: ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS

## Abstract

Nucleic acids encoding B7-1 and B7-2 molecules which bind CD28 or CTLA4 are described. These structural forms correspond to naturally-occurring alternatively spliced forms comprising cytoplasmic and signal peptide domains of T cell costimulatory molecules or variants thereof which can be produced by standard recombinant DNA techniques. These T cell costimulatory molecules can be used to identify agents which stimulate the express of alternative forms of costimulatory molecules and to identify components of the signal transduction pathway which results in costimulation of T cells.

## Claims

### Claim 1

An isolated nucleic acid molecule consisting of the nucleotide sequence of SEQ ID NO:1.

### Claim 2

An isolated nucleic acid molecule consisting of the nucleotide sequence of SEQ ID NO:4.

### Claim 3

An isolated nucleic acid molecule consisting of the nucleotide sequence of SEQ ID NO:8.

### Claim 4

An isolated nucleic acid molecule consisting of the nucleotide sequence of SEQ ID NO:10.

### Claim 5

An isolated nucleic acid molecule consisting of the nucleotide sequence of SEQ ID NO:12.

### Claim 6

An isolated nucleic acid molecule consisting of the nucleotide sequence of SEQ ID NO:14.

### Claim 7

An isolated nucleic acid molecule consisting of the nucleotide sequence of SEQ ID NO:62.

### Claim 8

An isolated nucleic acid molecule consisting of the nucleotide sequence of SEQ ID NO:64.

### Claim 9

An isolated nucleic acid molecule encoding a B7-1 or B7-2 protein which binds to CD28 or CTLA4 and comprises an amino acid sequence of SEQ ID NO:2.

### Claim 10

An isolated nucleic acid molecule encoding a B7-1 or B7-2 protein which binds to CD28 or CTLA4 and comprises an amino acid sequence of SEQ ID NO:5.

### Claim 11

An isolated nucleic acid molecule encoding a B7-1 or B7-2 protein which binds to CD28 or CTLA4 and comprises an amino acid sequence of SEQ ID NO:9, and does not comprise an amino acid sequence corresponding to an immnunoglobulin variable region-like domain.

### Claim 12

An isolated nucleic acid molecule encoding a B7-1 or B7-2 protein which binds to CD28 or CTLA4 and comprises an amino acid sequence of SEQ ID NO:11.

### Claim 13

An isolated nucleic acid molecule encoding a B7-1 or 37-2 protein which binds to CD28 or CTLA4 and comprises an amino acid sequence of SEQ ID NO:15.

### Claim 14

An isolated nucleic acid molecule encoding a B7-1 or B7-2 protein which binds to CD28 or CTLA4 and comprises an amino acid sequence of SEQ ID NO:63, and does not comprise an amino acid sequence corresponding to an immunoglobulin constant region-like domain.

### Claim 15

An isolated nucleic acid molecule encoding a B7-1 or B7-2 protein which binds to CD28 or CTLA4 and comprises an amino acid sequence of SEQ ID NO:65.

### Claim 16

An isolated nucleic acid molecule consisting of the nucleotide sequence of SEQ ID NO:7.