# US 6,294,375 B1 · Microbiological pressurised gas control device

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- Number: US 6,294,375 B1
- Document ID: US6294375B1
- Status: Granted
- Kind: B1
- Application number: 08/930,675
- Filed: 1998-11-04
- Granted: 2001-09-25
- Earliest claimed: 1995-04-06
- Assignees: Ultra Propre Nutrition Industrie Recherche (U.N.I.R.)
- Inventors: Philippe Chevalier
- Web page: https://patentgrep.com/patent/US6294375B1

## CPC

- C12M 23/10: {Petri dish (crystallising dishes B01L3/06)}
- C12M 25/02: {Membranes; Filters (filters or filtration in general B01D24/00-B01D41/00)}
- C12M 29/24: {Recirculation of gas}
- C12M 33/12: {by pressure}
- C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS}

## Abstract

A device for microbiologically controlling a dust-laden pressurized gas, including dust collecting device in an inner cavity provided with a gas inlet and a gas outlet and having an internal pressure that is equal to the pressure of said dust-laden gas at least during the operation of said device.

## Claims

### Claim 1

Microbiological pressurised gas control device carrying dust particles comprising a casing forming an inner cavity: collection means to collect said dust particles placed in said inner cavity fitted with a gas inlet and outlet and in which the prevailing pressure is, at least during the operation of said device, equal to the pressure of said gas carrying dust particles; and a sonic nozzle downstream from said collection means and placed at said gas outlet of said cavity.

### Claim 2 (depends on 1)

The device in accordance with claim 1, wherein the volume of said cavity lying between said collection means and said gas outlet is of minimum size and in the region of 30 cm3.

### Claim 3 (depends on 2)

The device in accordance with claim 2, comprising a lid closing said casing and inner cavity in a sealed manner, said casing being provided with inner fittings to carry said collection means.

### Claim 4 (depends on 3)

The device in accordance with claim 3, wherein said collection means comprise a Petri dish placed in said inner cavity, containing a nutrient medium facing said inlet into said cavity and a perforated impaction plate placed above said Petri dish parallel to the latter.

### Claim 5 (depends on 4)

The device in accordance with claim 4, wherein said impaction plate comprises approximately 600 perforations leading towards the base of said Petri dish, said perforations having a diameter of approximately 0.4 mm.

### Claim 6 (depends on 5)

The device in accordance with claim 5, wherein the distance between the surface of the nutrient medium placed in said Petri dish and the position of said impaction plate is determined in relation to the sizes of said perforations of said impaction plate, such that said distance is between two times and six times the diameter of said perforations.

### Claim 7 (depends on 4)

The device in accordance with claim 4, wherein the distance between the surface of the nutrient medium placed in said Petri dish and the position of said impaction plate is determined in relation to the sizes of said perforations of said impaction plate, such that said distance is between two times and six times the diameter of said perforations.

### Claim 8 (depends on 1)

The device in accordance with claim 1, comprising a a lid closing said casing and inner cavity in a sealed manner, said casing being provided with inner fittings to carry said collection means.

### Claim 9 (depends on 8)

The device in accordance with claim 8, wherein said collection means comprise a Petri dish placed in said inner cavity, containing a nutrient medium facing said inlet into said cavity and a perforated impaction plate placed above said Petri dish parallel to the latter.

### Claim 10 (depends on 9)

The device in accordance with claim 9, wherein said impaction plate comprises approximately 600 perforations leading towards a base of said Petri dish, said perforations having a diameter of approximately 0.4 mm.

### Claim 11 (depends on 10)

The device in accordance with claim 10, wherein the distance between the surface of the nutrient medium placed in said Petri dish and the position of said impaction plate is determined in relation to the sizes of said perforations of said impaction plate, such that said distance is between two times and six times the diameter of said perforations.

### Claim 12 (depends on 9)

The device in accordance with claim 9, wherein the distance between the surface of the nutrient medium placed in said Petri dish and the position of said impaction plate is determined in relation to the sizes of said perforations of said impaction plate, such that said distance is between two times and six times the diameter of said perforations.