# US 6,294,368 B1 · Isolated human metalloprotease proteins, nucleic acid molecules encoding human protease proteins, and uses thereof

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- Number: US 6,294,368 B1
- Document ID: US6294368B1
- Status: Granted
- Kind: B1
- Application number: 09/813,819
- Filed: 2001-03-22
- Granted: 2001-09-25
- Earliest claimed: 2001-03-22
- Assignees: Applera Corporation
- Inventors: Gennady V. Merkulov; Jane Ye; Valentina Di Francesco; Ellen M. Beasley
- Web page: https://patentgrep.com/patent/US6294368B1

## CPC

- C12N 9/6489: {Metalloendopeptidases (3.4.24)}
- A01K 2217/05: Animals comprising random inserted nucleic acids (transgenic)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A01K: ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS

## Abstract

The present invention provides amino acid sequences of peptides that are encoded by genes within the human genome, the protease peptides of the present invention. The present invention specifically provides isolated peptide and nucleic acid molecules, methods of identifying orthologs and paralogs of the protease peptides, and methods of identifying modulators of the protease peptides.

## Claims

### Claim 1

An isolated nucleic acid molecule consisting of a nucleotide sequence selected from the group consisting of:
(a) a nucleotide sequence that encodes an amino acid sequence shown in SEQ ID NO:2;
(b) a nucleic acid molecule consisting of the nucleic acid sequence of SEQ ID No: 1;
(c) a nucleic acid molecule consisting of the nucleic acid sequence of SEQ ID No: 3; and
(d) a nucleotide sequence that is completely complementary to a nucleotide sequence of (a)-(c).

### Claim 2 (depends on 1)

A nucleic acid vector comprising a nucleic acid molecule of claim 1.

### Claim 3 (depends on 2)

A host cell containing the vector of claim 2.

### Claim 4 (depends on 3)

A process for producing a polypeptide comprising culturing the host cell of claim 3 under conditions sufficient for the production of said polypeptide, and recovering the peptide from the host cell culture.

### Claim 5

An isolated polynucleotide consisting of a nucleotide sequence set forth in SEQ ID NO:1.

### Claim 6

An isolated polynucleotide consisting of a nucleotide sequence set forth in SEQ ID NO:3.

### Claim 7 (depends on 2)

A vector according to claim 2, wherein said vector is selected from the group consisting of a plasmid, virus, and bacteriophage.

### Claim 8 (depends on 2)

A vector according to claim 2, wherein said isolated nucleic acid molecule is inserted into said vector in proper orientation and correct reading frame such that the protein of SEQ ID NO: 2 may be expressed by a cell transformed with said vector.

### Claim 9 (depends on 8)

A vector according to claim 8, wherein said isolated nucleic acid molecule is operatively linked to a promoter sequence.