# US 6,294,364 B1 · Human FAS

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- Number: US 6,294,364 B1
- Document ID: US6294364B1
- Status: Granted
- Kind: B1
- Application number: 09/261,907
- Filed: 1999-03-03
- Granted: 2001-09-25
- Earliest claimed: 1999-03-03
- Assignees: SmithKline Beecham Corporation
- Inventors: Derk J Bergsma; Conrad Chapman; Megan E Depiera; Catherine E Ellis; John Lonsdale; Jeffrey L Mooney
- Web page: https://patentgrep.com/patent/US6294364B1

## CPC

- C12N 9/1029: {transferring groups other than amino-acyl groups (2.3.1)}
- C12Q 1/25: involving enzymes not classifiable in groups C12Q1/26 {- C12Q1/66}
- A61K 38/00: Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00)
- A61K 48/00: Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
- G01N 2500/20: cell-free systems
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

Fatty Acid Synthase polypeptides and polynucleotides and methods for producing such polypeptides by recombinant techniques are disclosed. Also disclosed are methods for utilising Fatty Acid Synthase polypeptides and polynucleotides in therapy, and diagnostic assays for such.

## Claims

### Claim 1

An isolated polynucleotide which comprises the nucleotide sequence of SEQ ID NO: 1.

### Claim 2 (depends on 1)

An isolated polynucleotide of claim 1 which consists of the nucleotide sequence of SEQ ID NO: 1.

### Claim 3

An isolated polynucleotide comprising a nucleotide sequence encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:2.

### Claim 4 (depends on 3)

The isolated polynucleotide of claim 3 which is DNA.

### Claim 5 (depends on 3)

The isolated polynucleotide of claim 3 which is RNA.

### Claim 6

An isolated polynucleotide comprising nucleotides 156 to 7688 of the nucleotide sequence of SEQ ID NO:1.

### Claim 7

An isolated polynucleotide comprising a polynucleotide sequence which is fully complementary to a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:2.

### Claim 8 (depends on 7)

The isolated polynucleotide of claim 7 wherein said polynucleotide sequence is fully complementary to nucleotides 156 to 7688 of the nucleotide sequence of SEQ ID NO:1.

### Claim 9

An expression system comprising a polynucleotide capable of producing a polypeptide comprising the amino acid sequence of SEQ ID NO:2.

### Claim 10 (depends on 9)

A process for producing a recombinant host cell comprising transforming or transfecting a host cell in vitro with the expression system of claim 9 such that the host cell produces said polypeptide in vitro.

### Claim 11 (depends on 10)

A recombinant host cell produced by the process of claim 10.

### Claim 12 (depends on 11)

A cell membrane of a recombinant host cell of claim 11 expressing said polypeptide.

### Claim 13 (depends on 10)

A process for producing a polypeptide comprising culturing a recombinant host cell of claim 10 under conditions sufficient for the production of said polypeptide and recovering said polypeptide from the culture.