# US 6,294,348 B1 · TRAF inhibitors

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- Number: US 6,294,348 B1
- Document ID: US6294348B1
- Status: Granted
- Kind: B1
- Application number: 09/020,683
- Filed: 1998-02-09
- Granted: 2001-09-25
- Earliest claimed: 1995-08-17
- Assignees: Genentech, Inc.; Tularik Inc.
- Inventors: David V. Goeddel; Mike Rothe
- Web page: https://patentgrep.com/patent/US6294348B1

## CPC

- C07K 14/7151: {for tumor necrosis factor [TNF], for lymphotoxin [LT]}
- A61P 35/00: Antineoplastic agents
- C07K 14/4703: {Inhibitors; Suppressors}
- A61K 38/00: Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00)
- A61K 48/00: Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)

## Abstract

The invention concerns novel inhibitors of tumor necrosis factor receptor associated factor-(TRAF) mediated signal transduction. The invention encompasses the novel inhibitor proteins (I-TRAFs), nucleic acid encoding them, methods for their recombinant production, and their use in screening assays and as pharmaceuticals.

## Claims

### Claim 1

An assay for identifying a molecule capable of modulating the association of an I-TRAF and a TRAF comprising (a) incubating a mixture comprising said I-TRAF and TRAF with a candidate molecule and (b) detecting of the ability of the candidate molecule to modulate I-TRAF/TRAF association.

### Claim 2 (depends on 1)

The assay of claim 1 wherein said candidate molecule prevents or inhibits the dissociation of an I-TRAF/TRAF complex.

### Claim 3 (depends on 1)

The assay of claim 1 wherein said candidate molecule prevents the association of I-TRAF and TRAF.

### Claim 4 (depends on 1)

The assay of claim 1 wherein said candidate molecule is a small molecule.

### Claim 5 (depends on 1)

The assay of claim 1 wherein said TRAF comprises the TRAF domain of a native TRAF1, TRAF2 or TRAF3 polypeptide.

### Claim 6 (depends on 1)

The assay of claim 1 wherein said TRAF, I-TRAF and cellular protein are human.