P element derived vector and methods for its use
AssigneesThe Board of Trustees of the Leland Stanford Jr. University, Tosk, Inc.
InventorsPatrick Fogarty, Joseph Lipsick
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Abstract
Novel P element derived vectors and methods for their use to insert an exogenous nucleic acid into the genome of a target cell are provided. The subject vectors have a pair of P element transposase recognized insertion sites, e.g. 31 base pair inverted repeats, flanking at least two transcriptionally active genes. In practicing the subject methods, a vector of the subject invention is introduced into the target cell under conditions sufficient for transposition to occur. The subject methods find use in a variety of applications in which the insertion of an exogenous nucleic acid into the genome of a target cell is desired, e.g. include research, synthesis and therapeutic applications.
Claims24
A P element vector for introducing an exogenous nucleic acid into a target non-insect cell genome, said vector comprising:
- a pair of P element transposase recognized insertion sequences flanking at least two non-insect cell transcriptionally active expression modules each comprising a coding sequence and a promoter.
A P element vector for introducing an exogenous nucleic acid into a non-insect target cell genome, said vector comprising:
- a pair of P element derived 31 base pair inverted repeats flanking at least two non-insect cell transcriptionally active genes, wherein each of said transcriptionally active-expression modules each comprising a coding sequence that is expressed under intracellular conditions.
A method of inserting an exogenous nucleic acid into a non-insect target cell genome, said method comprising:
- introducing into said cell a P-element derived vector comprising said exogenous nucleic acid under conditions sufficient for transposition to occur;
- whereby said exogenous nucleic acid is inserted into said genome;
- with the proviso that said target cell is not an insect cell.
A kit for use in inserting an exogenous nucleic acid into a non-insect target cell, said kit comprising:
- a P element vector comprising a pair of P element transposase recognized insertion sequences flanking at least two non-insect cell transcriptionally active expression modules each comprising a coding sequence and a promoter.
Dependencies are inferred from the claim text.